通过比较沃顿果衍生中酶体干细胞的生长动力学和特征,这些干细胞使用不同的培养介质进行了扩张
Muhammad Najib Fathi Hassan1, Muhammad Dain Yazid1, Mohd Heikal Bin Mohd Yunus2
1Department of Tissue Engineering and Regenerative Medicine (DTERM), Faculty of Medicine, Universiti Kebangsaan Malaysia, Kuala Lumpur, Malaysia.
Cytotechnology
|December 23, 2024
概括
优化为沃顿果衍生的介质干细胞 (WJ-MSCs) 的细胞培养对于治疗应用至关重要. DMEM-LG+10% HPL介质提供了最高的WJ-MSC产量,而不会影响细胞特性.
科学领域:
- 干细胞生物学 干细胞生物学
- 生物技术是生物技术.
- 再生医学是一种再生医学.
背景情况:
- 沃顿的果衍生的介质干细胞 (WJ-MSCs) 由于其免疫调节和分化潜力,对治疗很有希望.
- 大规模的体外扩展对于WJ-MSC的治疗应用至关重要.
- 优化培养条件是最大限度地提高WJ-MSC产量和保持功能的关键.
研究的目的:
- 为优化WJ-MSCs的细胞培养条件.
- 为了评估WJ-MSC扩展的不同媒体配方.
- 描述扩展的WJ-MSCs的治疗潜力.
主要方法:
- 在各种介质中培养WJ-MSC,包括用FBS或HPL补充的DMEM-LG,以及商业无血清介质.
- 细胞形态,生长动力学和产量在不同的播种密度下进行了评估.
- 评估了免疫表型,三系差异化和免疫抑制特性.
主要成果:
- DMEM-LG+10% HPL,商业介质1和商业介质2支持更高的细胞产量和更快的种群倍增时间.
- 在测试的最低种植密度下,DMEM-LG+10% HPL显示出最高的细胞产量.
- 所有测试的介质都支持MSC标记物的WJ-MSC表达,分化能力和免疫抑制功能.
结论:
- DMEM-LG+10% HPL是WJ-MSC扩张的最佳介质,最大限度地提高细胞产量,同时保持基本特征.
- 需要进一步研究使用生物反应器或含有这种介质的多层水瓶进行大规模WJ-MSC扩张.
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