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纸覆盖剂对从人类剥皮的叶子牙中分离出来的中细胞干细胞的细胞毒性影响

Bahareh Nazemi Salman¹, Ehsan Saburi², Mahtab Mohammadi Gheidari³

¹Dept. of Pediatrics, School of Dentistry, Zanjan University of Medical Sciences, Zanjan, Iran.

Journal of dentistry (Shiraz, Iran)

|December 23, 2024

概括

矿物三氧化物聚合物 (MTA),生物,丰富混合物 (CEM) 水泥和硫酸铁 (FS) 对干细胞具有相似的细胞毒性. 在较低度和更长的暴露时间下,生物相容性得到改善.

科学领域:

生物材料科学生物材料科学
牙科材料牙科材料
恢复性牙科恢复性牙科

背景情况:

在一次性牙中,脉冲切除术的成功取决于脉封闭剂 (PCA).
通常使用的PCA包括矿物三氧化物聚合物 (MTA),生物,丰富混合物 (CEM) 水泥和硫酸铁 (FS).
之前的评估主要关注这些药物的临床成功.

研究的目的:

为了比较四种常见PCA的体外细胞毒性.
评估不同度和暴露时间对细胞活力的影响.
为了评估PCAs与人体脱皮的叶状牙衍生的间酶体干细胞 (SHEDs) 相比.

主要方法:

人类脱皮的叶状牙衍生的介质干细胞 (SHED) 暴露于MTA,Biodentine,CEM水泥和FS.
使用甲基 thiazolyl tetrazolium (MTT) 试验来确定细胞活力.
五种不同度的暴露时间分别为24小时和72小时.

主要成果:

细胞毒性通常随着PCA度的降低而降低.
所有测试度在72小时显示出比24小时更高的生物相容性 (p<0.0001).
在任何时间点 (p>0.05) 中,在测试的PCA中都没有观察到细胞毒性的显著差异.

结论:

MTA,Biodentine,CEM水泥和FS的细胞毒性是可以比较的.
降低度和延长暴露时间提高了这些PCA的生物相容性.
MTA和Biodentine显示出最高的生物相容性,其次是FS和CEM水泥.

关键词:

落叶牙是指落叶牙的一种牙.牙纸的封闭方式干细胞是干细胞.牙的牙是一个牙.

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