用合成基因电路编程哺乳动物细胞聚合物的延长
Josquin Courte1, Christian Chung1, Naisargee Jain1
1Eli and Edythe Broad CIRM Center for Regenerative Medicine and Stem Cell Research, Keck School of Medicine, University of Southern California, Los Angeles, CA, USA.
bioRxiv : the preprint server for biology
|December 23, 2024
概括
研究人员设计了合成遗传电路来控制组织形状,从而使哺乳动物细胞集中的编程体积轴延长成为可能. 这种计算和体外方法推进了合成形态生成,用于创建多样化的组织架构.
科学领域:
- 合成生物学 合成生物学
- 发育生物学 发展生物学
- 生物物理学的生物物理.
背景情况:
- 合成形态生成旨在通过工程遗传电路来控制组织发育.
- 以前的方法通过粘附成功控制了细胞重组,但在电路识别,结构维度和效应器多样性方面存在局限性.
- 优化这些方法对于推进可编程组织工程至关重要.
研究的目的:
- 通过控制增殖,组织流动性和细胞-细胞信号来计算识别体积轴延长的遗传电路.
- 在实验室中在哺乳动物细胞聚合物中实现这些设计的电路.
- 为未来的电路开发,将in silico和in vitro发现整合到一个统一的形态空间中.
主要方法:
- 识别控制增殖,组织流动性和细胞对细胞信号的遗传回路的计算框架.
- 使用哺乳动物细胞聚合物的体外实验,以确定组织生长和流动性的效应因子.
- 构建和实施完整的合成遗传电路,以诱导可测量的组织变形.
主要成果:
- 通过计算方法成功识别了用于体积轴延长的遗传电路.
- 实验室实验证明了对哺乳动物细胞聚合物的组织生长和流动性的控制.
- 工程信号模块导致可测量的,轴依赖的组织变形,验证设计的电路.
结论:
- 开发的 in vitro/in silico管道为设计,选和实施合成遗传电路的形态发生提供了强大的方法.
- 这种方法可以编程特定的组织形状,进步合成形态发生的领域.
- 该研究为创建多样化和复杂的用户定义的组织架构打开了道路.
相关概念视频
02:55Synthetic Biology
4.7K
Synthetic biology is an interdisciplinary science that involves using principles from disciplines such as engineering, molecular biology, cell biology, and systems biology. It involves remodeling existing organisms from nature or constructing completely new synthetic organisms for applications such as protein or enzyme production, bioremediation, value-added macromolecule production, and the addition of desirable traits to crops, to name a few.
Golden rice
Golden rice is a genetically modified...
Golden rice
Golden rice is a genetically modified...
4.7K
01:20The Central Dogma
20.5K
The central dogma explains the flow of genetic information from DNA nucleotides to the amino acid sequence of proteins.
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
RNA is the Missing Link Between DNA and Proteins
In the early 1900s, scientists discovered that DNA stores all the information needed for cellular functions and that proteins perform most of these functions. However, the mechanisms of converting genetic information into functional proteins remained unknown for many years. Initially, it was believed that a single gene is...
20.5K
02:11Reporter Genes
11.2K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.2K