Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

Generation of Straight or Branched Actin Filaments01:14

Generation of Straight or Branched Actin Filaments

2.9K
The straight or branched structure formation of actin filaments is controlled by nucleating proteins such as the formins and Arp2/3 complex. Formin-mediated assembly results in straight filaments, whereas Arp2/3 protein complex-mediated assembly results in branched actin filaments.
Arp2/3 Complex
Arp2/3 complex is a seven-subunit complex consisting of two proteins similar to actin- Arp2 and Arp3, and five other subunits that help keep Arp2 and Arp3 inactive. When required, the complex is...
2.9K
Formation of Higher-order Actin Filaments01:11

Formation of Higher-order Actin Filaments

3.0K
The polymerization of G-actin monomers into filamentous F-actin is a multi-step process. Once the F-actins are formed, they can bundle together in different arrangements to form higher-order networks and regulate cellular functions. Common examples include the formation of lamellipodia and filopodia at the cell's leading edge by actin reorganization in a migrating cell. The microvilli on the brush border epithelial cells are also formed through the F-actin network.
The high-order actin...
3.0K
Introduction to Actin01:26

Introduction to Actin

4.9K
Actin is a highly conserved cytoskeletal protein found abundantly in eukaryotic cells. It constitutes 10% weight of the total cellular protein in muscle cells, while in non-muscle cells, it is lower and makes up around 1–5 percent of the total cell protein. Actin found in the unicellular amoebae and complex multicellular animals is around 80% similar, demonstrating their conservation over a billion years of evolution.  Actin coding genes are conserved within species and across...
4.9K
Actin Polymerization01:42

Actin Polymerization

6.3K
Actin polymerization occurs through the head-to-tail association of binding sites on monomeric actin or G-actin to form filamentous or F-actin. The polymerization can be divided into three phases ̶  nucleation, elongation, and steady-state phase.
The nucleation phase involves forming a stable nucleus consisting of three actin monomers to form a new actin filament. Actin-binding proteins such as formins and Arp2/3 complex help filament growth post-nucleation. The Formins form straight...
6.3K
Actin Filament Depolymerization01:19

Actin Filament Depolymerization

3.0K
Actin filaments (F-actin) are composed of actin subunits. The dissociation of actin monomers can occur from either end of F-actin. The rate of dissociation is faster from the minus-end or the pointed end, where the actin subunits exist with a bound ADP, together known as ADP-actin. The depolymerization of F-actin is aided by proteins, including the actin-depolymerizing factor (ADF) and cofilin family of proteins, gelsolin, and glia maturation factor (GMF).
In F-actin, the ADF/cofilin proteins...
3.0K
Actin Polymerization and Cell Motility01:13

Actin Polymerization and Cell Motility

5.1K
Actin is a family of globular proteins that are highly abundant in eukaryotic cells. It makes up approximately 1-5% of total cell protein concentration. Actin monomers polymerize to form a complex network of polarized filaments, the actin cytoskeleton, that plays a crucial role in many cellular processes, including cell motility, division, endocytosis, and metastasis of cancer cells.
Actin cytoskeleton dynamics can produce pushing, pulling, and resistance forces that help the cell to migrate....
5.1K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Differences in coronary disease severity at time of acute coronary syndrome between Middle East and North America.

Cardiovascular diagnosis and therapy·2026
Same author

Phase separation as a tunable regulator of canonical gene regulatory motifs.

Journal of the Royal Society, Interface·2026
Same author

Association of bidi smoking and tobacco-use patterns with cancer risk in India: a multicentre unmatched case-control study (2024-2025).

The Lancet regional health. Southeast Asia·2026
Same author

Incidental Radiation Exposure to the Internal Mammary Lymph Nodes in Breast Cancer Patients Undergoing Intensity-Modulated Radiation Therapy: A Retrospective Analysis.

The Gulf journal of oncology·2026
Same author

Actin dynamics: Filament end remodeling drives rapid depolymerization.

Current biology : CB·2026
Same author

To Intervene or Not to Intervene: Rethinking Non-culprit Vessels in Acute Coronary Syndrome.

Journal of cardiothoracic and vascular anesthesia·2026

相关实验视频

Updated: Jun 4, 2025

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics
09:10

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

Published on: August 25, 2022

3.0K

一个通用的理论框架来研究多组分actin动力学.

Mintu Nandi1, Shashank Shekhar2, Sandeep Choubey3

  • 1Department of Chemistry, Indian Institute of Engineering Science and Technology, Shibpur, Howrah 711103, India.

bioRxiv : the preprint server for biology
|December 23, 2024
PubMed
概括

我们开发了一种动力模型,以了解多个actin结合蛋白如何调节actin丝长度动态. 这一框架有助于解释实验数据,并指导未来关于细胞活性调节的研究.

更多相关视频

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy
08:44

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy

Published on: July 20, 2022

3.3K
Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
08:02

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Published on: May 5, 2022

2.5K

相关实验视频

Last Updated: Jun 4, 2025

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics
09:10

Reconstituting and Characterizing Actin-Microtubule Composites with Tunable Motor-Driven Dynamics and Mechanics

Published on: August 25, 2022

3.0K
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy
08:44

Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence TIRF Microscopy

Published on: July 20, 2022

3.3K
Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles
08:02

Using Microfluidics and Fluorescence Microscopy to Study the Assembly Dynamics of Single Actin Filaments and Bundles

Published on: May 5, 2022

2.5K

科学领域:

  • 细胞生物学 细胞生物学
  • 生物物理学的生物物理.
  • 生物化学 生物化学

背景情况:

  • 动氨酸丝长度对于细胞功能至关重要.
  • 数以百计的动因结合蛋白调节了动因动态.
  • 目前对多组件调节的理解是有限的.

研究的目的:

  • 开发一个理论框架,以理解多组分调节的行为动力学.
  • 为了提供一个机械的理解,多个actin-binding蛋白质如何集体控制actin丝长度.
  • 为了使实验数据对actin动态的解释.

主要方法:

  • 提议了一种一般动力学模型来调节actin线程.
  • 导出了导线长度分布时刻的封闭式表达式.
  • 该模型捕捉了多个调节蛋白的综合作用.

主要成果:

  • 动力模型成功地描述了多个活性蛋白结合蛋白的联合作用.
  • 提供了导线长度分布的依赖时间和稳定状态时刻的表达式.
  • 该框架可以区分各种监管机制.

结论:

  • 拟议的动力学模型为actin动力学提供了一个统一的理论框架.
  • 这种方法有助于解释实验数据和未来的研究方向.
  • 进步了解蛋白质如何在体内集体调节活性丝长度的理解.