相关实验视频
Updated: Jun 4, 2025

06:04
Murine Dermal Fibroblast Isolation by FACS
Published on: January 7, 2016
21.0K
皮肤纤维细胞稳定状态的改变有助于治愈结果
Yaqing Huang1,2, Nuoya Wang2,3, Hao Xing4,2
1Department of Pathology, Yale University, New Haven, CT 06520, USA.
bioRxiv : the preprint server for biology
|December 23, 2024
概括
从纤维细胞中去除Thrombospondin-2 (TSP-2) 增强了它们的再生功能. 这会通过促进细胞迁移和增殖,改善伤口愈合和增加神经发生.
科学领域:
- 细胞生物学 细胞生物学
- 组织修复机制 组织修复机制
- 纤维细胞异质性 纤维细胞异质性
背景情况:
- 纤维细胞对于组织发育和修复至关重要,具有多种功能.
- 纤维细胞产生的蛋白质Thrombospondin-2 (TSP-2) 抑制血管生成,并损害组织的修复.
研究的目的:
- 研究纤维细胞中TSP-2缺乏的功能和分子后果.
- 探索TSP-2缺乏纤维细胞在促进组织再生方面的潜力.
主要方法:
- 野生型 (WT) 和TSP2淘汰 (TSP2KO) 皮肤纤维细胞的单细胞测序.
- 在信号通路的分析.
- 在CRISPR/Cas9基因编辑中创建TSP2KO NIH3T3细胞系.
- 免疫覆盖和功能测定 (迁移,增殖).
主要成果:
- TSP2KO纤维细胞显示Sox10+ Schwann前体细胞的丰富,纤维化相关亚群的减少.
- 在分析中发现,TSP2KO细胞中增强了亲生存信号 (WNT,TGF-β,PDGF-β) 和降低了BMP4反应.
- TSP2KO NIH3T3细胞表现出增加的迁移,增殖,提升的亲再生分子 (TGF-β3,Wnt4) 和核β-catenin丰富.
结论:
- 缺少TSP-2会改变纤维细胞的异质性,促进一个亲再生的表型.
- 缺乏TSP-2的纤维细胞具有增强的迁移和繁殖能力.
- 这些发现表明,TSP-2缺乏的纤维细胞可能会改善伤口愈合和神经发生.
相关概念视频
Clinical Applications of Epidermal Stem Cells
2.7K
Epidermal stem cells (EpiSCs) are mainly located at the basal layer of the epidermis. These cells repair minor injuries of the skin and replace dead skin cells. However, EpiSCs’ cannot heal severe wounds such as major burns or those from diabetes or hereditary disorders. In such cases, culturing the epidermal stem cells from the patient is possible and has yielded successful treatment options, such as laboratory-grown skin grafts. These grafts are synthesized using a patient’s own...
2.7K
Introduction to Fibroblasts
3.0K
Rudolph Virchow discovered spindle-shaped cells called fibroblasts in 1858. Inactive fibroblasts, called fibrocytes, become activated by various stimuli, such as growth factors and inflammatory cytokines. Activated fibroblasts play a crucial role in wound healing, inflammation, formation of new blood vessels, and cancer progression. Uncontrolled activation of fibroblasts results in fibrosis, the excess deposition of fibrous tissue, which can lead to scarring and affect normal organs. This...
3.0K
Renewal of Skin Epidermal Stem Cells
2.5K
The skin is divided into epidermis, dermis, and hypodermis, the skin's outermost, middle, and inner layers. The human epidermal layer regularly undergoes renewal, where old, dead cells are replaced by new cells. Epidermal stem cells or EpiSCs divide and differentiate to restore the lost cells. For the renewal process, some EpiSCs continuously self-renew. In contrast, few others differentiate into transit-amplifying cells, which later form prickle or spinous cells, followed by granular...
2.5K
Phases of Wound Repair
5.9K
Following injury, the integrity of the injured tissues must be reestablished. For example, in skin tissue, wound repair involves coordination among resident skin cells, blood mononuclear cells, extracellular matrix, growth factors, and cytokines to complete the healing cascade.
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
Formation of Blood Clot
In case of deep injuries, trauma to blood vessels results in blood loss. In the meantime, phospholipids released from the ruptured endothelial cellular membrane are converted into arachidonic...
5.9K
Overview of Regeneration and Repair
3.9K
Regeneration and repair processes are critical in healing damages caused by injury, disease, and aging. In regeneration, the damaged tissue is entirely replaced with new growth that restores the original architecture and function. In contrast, tissue repair usually results in a fixed tissue architecture involving scar formation. Scars generally do not reestablish tissue function and may also exhibit structural abnormalities at the injury site.
Regeneration
All animals have varying degrees of...
Regeneration
All animals have varying degrees of...
3.9K

