Jove
Visualize
联系我们
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关概念视频

RNA-seq03:21

RNA-seq

9.8K
RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while...
9.8K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Lysosomal down-regulation of the mu opioid receptor is opposed by the Retromer complex.

Science advances·2026
Same author

Transcriptomic diversity of amygdalar subdivisions across humans and nonhuman primates.

Science advances·2025
Same author

Human brain cell types shape host-rabies virus transcriptional interactions revealing a preexisting pro-viral astrocyte subpopulation.

bioRxiv : the preprint server for biology·2025
Same author

AnnSQL: a Python SQL-based package for fast large-scale single-cell genomics analysis using minimal computational resources.

Bioinformatics advances·2025
Same author

Transcriptomic diversity of amygdalar subdivisions across humans and nonhuman primates.

bioRxiv : the preprint server for biology·2024
Same author

Ascertaining cells' synaptic connections and RNA expression simultaneously with barcoded rabies virus libraries.

Nature communications·2022
Same journal

Complex Indel Detection: A Simulation-Based Framework and Parsing with FreeBayes.

bioRxiv : the preprint server for biology·2026
Same journal

Emulating the gingival-tooth interface during bacterial, fungal, and viral infection in a microphysiological model of the human oral cavity.

bioRxiv : the preprint server for biology·2026
Same journal

Local SNP-explained methylation variation reveals genetically anchored and exposure-associated methylation architecture in the human brain.

bioRxiv : the preprint server for biology·2026
Same journal

Perinatal Semaglutide Treatment Improves Maternal Health and Mitigates Offspring Metabolic Dysfunction in a Mouse Model of Maternal Obesity.

bioRxiv : the preprint server for biology·2026
Same journal

Pervasive cryptic selection in the human noncoding genome.

bioRxiv : the preprint server for biology·2026
Same journal

Secreted ORF8 reprograms macrophages to enhance SARS-CoV-2 infection of lung epithelial cells.

bioRxiv : the preprint server for biology·2026
查看所有相关文章

相关实验视频

Updated: Jun 4, 2025

Infinium Assay for Large-scale SNP Genotyping Applications
13:33

Infinium Assay for Large-scale SNP Genotyping Applications

Published on: November 19, 2013

38.9K

AnnSQL:一个基于Python SQL的包,用于快速大规模的单细胞基因组学分析,使用最小的计算资源.

Kenny Pavan, Arpiar Saunders

    bioRxiv : the preprint server for biology
    |December 23, 2024
    PubMed
    概括
    此摘要是机器生成的。

    AnnSQL是一个新的Python包,它使用SQL快速高效地分析大型单细胞基因组学数据集. 这个工具使复杂的单细胞分析在个人电脑上可访问,显著减少计算障碍.

    更多相关视频

    An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
    12:10

    An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

    Published on: November 30, 2014

    13.3K
    An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
    10:00

    An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

    Published on: May 23, 2018

    17.5K

    相关实验视频

    Last Updated: Jun 4, 2025

    Infinium Assay for Large-scale SNP Genotyping Applications
    13:33

    Infinium Assay for Large-scale SNP Genotyping Applications

    Published on: November 19, 2013

    38.9K
    An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus
    12:10

    An Experimental and Bioinformatics Protocol for RNA-seq Analyses of Photoperiodic Diapause in the Asian Tiger Mosquito, Aedes albopictus

    Published on: November 30, 2014

    13.3K
    An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing
    10:00

    An Ultrahigh-throughput Microfluidic Platform for Single-cell Genome Sequencing

    Published on: May 23, 2018

    17.5K

    科学领域:

    • 计算生物学 计算生物学
    • 基因组学就是基因组学.
    • 生物信息学是一种生物信息学.

    背景情况:

    • 单细胞基因组学技术正在迅速发展,产生大量的数据集.
    • 分析这些大规模数据集需要高效和可访问的软件工具.
    • 现有的工具可能是计算密集型,限制了许多研究人员的可访问性.

    研究的目的:

    • 介绍AnnSQL,这是一个用于分析单细胞基因组学数据的Python包.
    • 展示性能提升和减少计算资源需求.
    • 降低个人电脑上大规模单细胞分析的计算障碍.

    主要方法:

    • 开发了AnnSQL,这是一个使用过程中的DuckDB引擎的Python包.
    • 在AnnSQL.SQL中构建了一个基于AnnData的数据库.
    • 在440万个细胞单核RNA-seq数据集上比较AnnSQL和AnnData的性能.

    主要成果:

    • AnnSQL为解析单细胞基因组学数据集提供了数量级的性能改进.
    • 在笔记本电脑上,在大数据集上的AnnSQL操作在几分钟内完成.
    • 在高性能计算集群上,同等的AnnData操作失败或显著减速.
    • AnnSQL展示了变革性的运行时间改进.

    结论:

    • AnnSQL为大规模单细胞基因组学分析提供了一个计算效率高且易于使用的解决方案.
    • 该套件可以在个人电脑上进行复杂的分析,民主化对单细胞数据的访问.
    • AnnSQL为未来的单细胞工作流提供了一个有前途的计算基础设施,跨越各种全基因组测量.