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维生素B12宏复合物的干扰:对低维生素和高维生素血的有效检测和正确解释

José A Delgado¹, María I Pastor¹, Gemma Costa¹

¹Department of Laboratory Medicine, Hospital Universitari Son Espases, Palma, Spain.

Advances in laboratory medicine

|December 23, 2024

概括

聚乙烯甘醇 (PEG) 沉有助于识别宏B12,这是导致虚假高维生素B12水平的原因. 建立后PEG参考间隔和恢复率可以更准确地评估真正的维生素B12状态.

科学领域:

临床化学临床化学
生物化学生物化学
实验室医学实验室医学

背景情况:

宏观B12是一种高分子量维生素B12的形式,可以导致错误地提高总维生素B12度 (维生素B12过高血症).
聚乙烯甘醇 (PEG) 沉是一种常见的方法来去除高分子量物质,但可以发生自由维生素B12的共同沉.
准确地解释维生素B12状态需要考虑宏观B12的潜在干扰和沉方法的影响.

研究的目的:

确定维生素B12的PEG后参考间隔 (RIs).
比较确定宏观B12存在的方法.
评估PEG后维生素B12恢复和调整后RI的综合实用性,以评估真正的维生素B12状态.

主要方法:

一项前性研究分析了488个健康个体的血清样本,以确定PEG后恢复和RI.
对297个病例的回顾性分析评估了对宏观B12怀疑的标准.
维生素B12度在PEG沉之前和之后使用Alinity i平台进行测量.

主要成果:

后PEGRI范围在118至506 pmol/L之间,恢复率在60-107%之间.
在估计干扰的标准之间发现了统计学上显著的差异,但方法显示出良好的一致性.
在27例病例中,宏B12与真正的维生素B12过高血症共存;没有观察到与维生素B12缺乏的同时发生.

结论:

实验室报告应包括总维生素B12,PEG后维生素B12度和调整后的RI.
与PEG后值一起报告维生素B12恢复百分比可以提高临床解释.
这些综合数据有助于更准确地评估患者的真实维生素B12状态.

关键词:

B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 B12 生物标志物生物标志物科巴拉胺是什么意思干扰干扰是干扰的大分子分子是巨分子.参考时间间隔的参考时间间隔.

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