乙醇胺诱导的微分区组合对于菌核酸菌的毒性是必要的
Dana S Franklin1, Yi-Wei Chen2, Yimin Chen2
1Molecular Biology Institute, University of California, Los Angeles, California, USA.
Fusobacterium nucleatum使用细菌微分区 (BMC) 来代谢乙醇胺,这是由EutV-EutW系统调节的过程. 这种BMC介导的新陈代谢对细菌毒性至关重要,并在小鼠模型中导致过早分娩.
科学领域:
- 微生物学 微生物学
- 细胞生物学 细胞生物学
- 病变的发生和发病.
背景情况:
- Fusobacterium nucleatum是一种口腔病原体,与不良妊娠结果有关.
- 了解F. nucleatum如何适应宿主对于其致病性至关重要.
- 乙胺 (EA) 是一种营养来源,细菌通过专门的有机细胞利用.
研究的目的:
- 研究F. nucleatum.中的细菌微分区 (BMC) 的组装,调节和功能.
- 阐明BMCs在乙醇胺利用和毒性中的作用.
- 确定控制乙醇胺反应BMC形成的调节机制.
主要方法:
- 乙醇胺利用 (eut) 位置的基因表达分析.
- 删除突变的构建和分析 (例如,eutV,eutN,eutL/eutM/eutM).
- 质谱测量用于BMC蛋白质识别.
- 评估BMC的形成和乙醇胺的利用.
- 在早产的小鼠模型中评估细菌毒性.
主要成果:
- 乙醇胺显著增加了野生型F. nucleatum的eut基因表达和BMC形成.
- EutV-EutW两组系统在对乙醇胺的反应中通过转录来调节BMC的形成.
- BMC外蛋白 (EutL,EutM1,EutM2,EutN) 对于BMC组装和乙醇胺利用至关重要.
- 在BMC形成中有缺陷的突变体表现出乙醇胺利用率受损和生长缺陷.
- 在早产小鼠模型中,破坏BMC形成 (eutN突变) 显著降低了毒性.
结论:
- BMC介导的乙醇胺代谢对F.核病毒性至关重要.
- 欧特维-欧特维TCS控制BMC的形成,以回应乙醇胺的可用性.
- 在宿主环境中,F. nucleatum的适应性和病原性中,包括怀孕期间,BMCs起着至关重要的作用.
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