相关实验视频
Updated: Jun 4, 2025

11:15
Development of a 3D Graphene Electrode Dielectrophoretic Device
Published on: June 22, 2014
11.9K
液体金属电极启用级联在芯片上的介电电离分离大尺寸范围颗粒
Huichao Chai1, Liang Huang2, Junwen Zhu1
1State Key Laboratory of Precision Measurement Technology and Instrument, Department of Precision Instrument, Tsinghua University, Beijing 100084, P. R. China. wwh@tsinghua.edu.cn.
Lab on a chip
|December 23, 2024
概括
这项研究引入了一种新的微流体装置,用于使用介电泳 (DEP) 进行无标签的颗粒分离. 级联设计有效地按大小分离颗粒,为复杂的生物样本提供了一个强大的平台.
科学领域:
- 生物医学工程 生物医学工程
- 微流体学 微流体学
- 颗粒分离技术 颗粒分离技术
背景情况:
- 在复杂的生物样本中分离不同的粒子大小至关重要,但具有挑战性.
- 现有的无标签过电泳 (DEP) 方法在微流体装置中难以有效地接触各种大小的粒子.
- 存在对集成系统的需求,这些系统可以在没有标签的情况下处理广泛的颗粒大小范围.
研究的目的:
- 提出和验证一种新的微流体装置概念,用于无标签,以大小为基础的颗粒分离.
- 为提高分离效率,将被动流量分成与累积介电泳 (DEP) 偏移相结合.
- 为了证明该设备在分离广泛的粒子大小的能力,模仿生物复杂性.
主要方法:
- 一个级联式微流体设计,采用交替的DEP偏移段和被动绕道流量分数出口.
- 在DEP段内使用侧墙液体金属电极来产生尺寸特定的DEP力.
- 采用顺序流量分成机制,逐渐隔离颗粒大小范围.
主要成果:
- 成功地从混合样本中分离了五种不同的颗粒大小,从16微米到0.5微米,从混合样本中分离出来.
- 通过通过连续的绕道出口来减少尺寸范围来证明粒子的顺序分离.
- 验证了该概念在处理复杂混合物时的有效性,模拟了血液成分.
结论:
- 拟议的集成微流体系统有效地使用DEP和流量分成的组合来分离大尺寸范围内的颗粒.
- 这种无标签的方法提供了一个多功能和强大的平台,用于在各种生物医学应用中分离多种粒子类型.
- 级联设计为复杂的生物流体中高分辨率颗粒分离提供了有希望的解决方案.
相关概念视频
Electrodeposition
597
Electrodeposition is a technique used to separate an analyte from interferents by electrochemical processes. Here, the analyte is a metal ion that can be deposited on an electrode immersed in the sample solution. The electrochemical setup consists of an anode and a cathode. When an electric current is applied to the setup, oxidation occurs at the anode. At the cathode, which consists of a large metal surface, metal ions undergo reduction and deposit onto the surface.
Electrodeposition can...
Electrodeposition can...
597
Capillary Electrophoresis: Applications
332
Capillary electrophoretic separations offer various modes, each with unique applications. These modes include capillary zone electrophoresis, capillary gel electrophoresis, capillary array electrophoresis, capillary isoelectric focusing, capillary isotachophoresis, micellar electrokinetic chromatography, and capillary electrochromatography.
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
Capillary zone electrophoresis (CZE) separates ionic components based on their electrophoretic mobility. It has been used to separate proteins, amino acids,...
332
Capillary Electrophoresis: Instrumentation
180
Capillary electrophoresis instrumentation typically consists of several key components. A high-voltage power supply generates the electric field necessary for the separation by connecting to an anode (the positively charged electrode) and a cathode (the negatively charged electrode) located in buffer reservoirs at each end of the capillary tube. The system includes a sample vial, a fused silica capillary tube coated with polyimide for mechanical strength through which the sample components...
180
Electrophoresis: Overview
1.5K
Electrophoresis is a powerful analytical separation technique that relies on the differential migration of charged species when subjected to an electric field. The core strength of electrophoresis lies in its ability to separate high-molecular-weight species in complex mixtures. It has found widespread use in biochemistry, molecular biology, and analytical chemistry, allowing the separation of compounds like amino acids, nucleotides, carbohydrates, and proteins with excellent resolution.
There...
There...
1.5K

