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Updated: Jun 4, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
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高效的转酶催化异结合

Simon J de Veer1, David J Craik1, Fabian B H Rehm1,2

  • 1Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, Queensland 4072, Australia.

Journal of the American Chemical Society
|December 23, 2024
PubMed
概括

工程化阿斯巴拉基尼酶OaAEP1可使用内部的2,3-氨基酸残留物进行直接的异结合. 这种酶在温和条件下促进各种改和蛋白质融合,扩大合成生物学应用.

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Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
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