Jove
Visualize
联系我们

相关概念视频

Tagging and Fusion Proteins01:24

Tagging and Fusion Proteins

6.6K
Proteins are involved in several cellular processes and biochemical reactions. Analyzing a specific protein of interest requires it to be isolated from the other proteins in the cell. This is achieved by overexpressing the specific gene in a suitable host to produce large quantities of the target protein. A tag or label is recombined with the gene to produce a fusion protein containing the target protein and the tag. The tags on these fusion proteins can then be used for easy detection and...
6.6K

您也可能阅读

相关文章

通过共同作者、期刊和引用图与本文相关的文章。

排序
Same author

Computer-Aided Design of <i>Plasmodium chabaudi</i>-Derived Peptides with Dual Antibiofilm and Anti-inflammatory Activities.

ACS medicinal chemistry letters·2026
Same author

Site-specific protein and peptide modification by redeploying an asparaginyl ligase for noncanonical reactions.

Nature protocols·2026
Same author

Enzyme-Assisted Synthesis and In Vitro Characterization of Bifunctional PCSK9 Inhibitors.

Chembiochem : a European journal of chemical biology·2026
Same author

Highly mutagenic continuous evolution in E. coli using a Φ29-based orthogonal replication system.

Nature biotechnology·2026
Same author

Rational Design of Plant-Derived Protein Ligases with Altered Substrate Specificity.

Biochemistry·2026
Same author

Progress and Challenges in the Biosynthesis and Chemical Synthesis of Lasso Peptides.

Journal of the American Chemical Society·2026
JoVE
x logofacebook logolinkedin logoyoutube logo
关于 JoVE
概览领导团队博客JoVE 帮助中心
作者
出版流程编辑委员会范围与政策同行评审常见问题投稿
图书馆员
用户评价订阅访问资源图书馆顾问委员会常见问题
研究
JoVE JournalMethods CollectionsJoVE Encyclopedia of Experiments存档
教育
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab Manual教师资源中心教师网站
使用条款与条件
隐私政策
政策

相关实验视频

Updated: Jun 4, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.7K

高效的转酶催化异结合

Simon J de Veer1, David J Craik1, Fabian B H Rehm1,2

  • 1Institute for Molecular Bioscience, Australian Research Council Centre of Excellence for Innovations in Peptide and Protein Science, The University of Queensland, Brisbane, Queensland 4072, Australia.

Journal of the American Chemical Society
|December 23, 2024
PubMed
概括

工程化阿斯巴拉基尼酶OaAEP1可使用内部的2,3-氨基酸残留物进行直接的异结合. 这种酶在温和条件下促进各种改和蛋白质融合,扩大合成生物学应用.

科学领域:

  • 生物化学
  • 合成生物学
  • 酵素学

背景情况:

  • 传统上,转酶会修改骨.
  • 适应酶的侧链修饰具有挑战性,通常需要特定的标签或条件.

研究的目的:

  • 开发一种使用工程酶选择性和蛋白质修饰的新方法.
  • 探索工程化阿斯巴拉基尼尔连接酶OaAEP1对异连接的功能.

主要方法:

  • 设计了阿斯巴拉基尼酶OaAEP1.
  • 使用2,3-diaminopropionic acid (Dap) 作为一个内部接受器残留物.
  • 在几乎中性pH下进行反应,没有添加剂.

主要成果:

  • OaAEP1通过内部Dap残留物直接催化,模仿天然基质.
  • 包括循环和蛋白质融合在内的多种异结合产物的有效形成.
  • 与L-和D-受体成功结合,包括混合双环结构.

结论:

  • 工程OaAEP1为异键形成提供了多功能和高效的工具.
  • 该酶使复杂的和蛋白质结构的选择性标记和构建成为可能.

更多相关视频

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

10.7K
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
12:05

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Published on: March 6, 2013

14.1K

相关实验视频

Last Updated: Jun 4, 2025

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy
08:34

OaAEP1-Mediated Enzymatic Synthesis and Immobilization of Polymerized Protein for Single-Molecule Force Spectroscopy

Published on: February 5, 2020

6.7K
Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation
11:09

Constructing Thioether/Vinyl Sulfide-tethered Helical Peptides Via Photo-induced Thiol-ene/yne Hydrothiolation

Published on: August 1, 2018

10.7K
Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies
12:05

Production of Disulfide-stabilized Transmembrane Peptide Complexes for Structural Studies

Published on: March 6, 2013

14.1K
  • OaAEP1扩大了酶性改的范围,包括非天然的氨基酸和立体异构体.