在小鼠中,在关节损伤后,创伤后过敏症和相关的外周敏感化
Ishraq Alshanqiti1,2,3, Hyeonwi Son4,5, John Shannonhouse4
1Program in Dental Biomedical Sciences, School of Dentistry, University of Maryland Baltimore, Baltimore, MD, United States.
Pain
|December 23, 2024
概括
关节 (TMJ) 的创伤会引起疼痛和焦虑. 虽然两性疼痛行为相似,但三神经敏感性显示出性别特异性差异,特别是在男性中.
科学领域:
- 神经科学是一个神经科学.
- 疼痛研究 疼痛研究
- 面生物学 面生物学
背景情况:
- 部部疾病 (TMD) 是一种常见的疼痛的面状况.
- 关节 (TMJ) 的创伤与痛苦的TMD的发展有关.
研究的目的:
- 研究关节创伤诱导的强迫口腔开口 (FMO) 对小鼠疼痛行为和三角管 nociceptor 敏感性的影响.
- 为了确定这些反应的性别依赖差异.
主要方法:
- 小鼠接受了强迫口腔开口 (FMO) 或假冒程序.
- 疼痛行为被评估使用·弗雷亚丝,小鼠笑容尺度,和开放场测试.
- 在体内GCaMP Ca2+对三角关节成像 (TG) 和对逆向标记的TMJ afferents进行了分析.
主要成果:
- FMO增加了机械疼痛,自发的类似疼痛的行为和类似焦虑的行为,没有观察到性别差异.
- 活体成像显示TG神经元活动和机械过敏度的增加取决于模式和性别,在男性中更为明显.
- 在男性和女性中,FMO增加了对寒冷,热和素刺激的Ca2+反应.
- 关节 afferent分析显示,表达CGRP和TRPV1的小型神经元增加,性别依赖程度适度.
结论:
- 关节损伤导致持续的创伤后过敏症.
- 三角质 nociceptors的外周敏感化是这种过敏症的基础.
- 敏感化过程表现出明显的性别依赖性特征.
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