细菌花生物虫 (Bacillus cereus biovar anthracis) 产生的毒素和囊会影响巨细胞和动物模型中的致病性
Treenate Jiranantasak1,2, Andrew P Bluhm1,2, Donald J Chabot3
1Spatial Epidemiology & Ecology Research Laboratory, Department of Geography, University of Florida, Gainesville, Florida, United States of America.
PLoS neglected tropical diseases
|December 23, 2024
概括
细菌花生物炭会引起类似炭病的疾病,并且比Bacillus anthracis.更具毒性. 斯特恩疫苗在动物模型中有效地保护动物免受Bacillus cereus biovar anthracis感染.
科学领域:
- 微生物学 微生物学
- 病原体生物学 病原体生物学
- 兽医医学 兽医医学 兽医医学
背景情况:
- 在非洲野生动物中,Bacillus cereus biovar anthracis (Bcbva) 会导致类似炭的疾病.
- Bcbva与Bacillus anthracis具有遗传相似性,包括毒素和囊编码等离子体.
- 与B. anthracis.相比,Bcbva病变的研究有限.
研究的目的:
- 为了研究Bcbva的分泌,毒素和囊合成.
- 为了比较Bcbva和B. anthracis的致病性在体外和体内.
- 为了评估斯特恩疫苗对Bcbva感染的疗效.
主要方法:
- 培养Bcbva和B. anthracis以评估生长和化.
- 测量保护性抗原 (PA) 分泌和聚-ƴ-D-胺酸 (PDGA) 囊生产.
- 使用小鼠巨细胞进行细胞化测定.
- 评估Galleria mellonella幼虫和几内亚猪模型中的毒性.
- 通过测量已接种疫苗的几内亚猪的抗PA和毒素中和抗体标位来评估疫苗的疗效.
主要成果:
- Bcbva的分泌速度比B. anthracis Ames.更快,更广泛.
- 在特定条件下,BCbva分泌的PA较少,但产生更多的PDGA囊.
- Bcbva 子表现出巨细胞内部化的减少.
- 在昆虫和豚鼠模型中,BCbva比B. anthracis Ames更具毒性.
- 严格疫苗接种显著增加了几内亚猪的抗PA和毒素中和抗体标位.
结论:
- 与B. anthracis. 相比,Bcbva表现出明显的致病特征.
- 斯特恩疫苗证明了对海豚中Bcbva诱导的炭样疾病的保护性有效性.
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