台式离心法:一种有效的方法来减少与脂血病相关的干扰在粗的脂血病样本?
James L Hall1, Henry C Carlton1, Kate E Shipman1,2
1Department of Clinical Chemistry, University Hospitals Sussex NHS Trust, Chichester, UK.
Annals of clinical biochemistry
|December 23, 2024
概括
台式离心法有效降低血清样本中的脂血,使其适合分析. 这种方法为超离心法提供了切实可行的替代方案,而不会对关键分析结果产生临床影响.
科学领域:
- 临床化学 临床化学
- 实验室医学 实验室医学
- 分析生物化学 分析生物化学
背景情况:
- 粗略的脂血样本会导致显著的分析错误,影响患者的护理.
- 传统的超离心法用于降低脂血症,但由于可访问性和成本受到限制.
- 测试台离心法被探索为一个实际的替代方案.
研究的目的:
- 为了评估基准离心法在减少血清样本中的脂血的有效性.
- 评估基板离心法对核心分析剂测量准确性的影响.
主要方法:
- 53个脂血性血清样本在18840克的温度下进行了离心,持续时间不同.
- 在离心离散前和之后测量了脂血水平.
- 在离心30分钟前和之后的样本上测量了核心分析物.
主要成果:
- 90%的样品在离心5分钟后达到理想或适当的脂血水平.
- 所有样本在离心30分钟后都适合分析.
- 离心后没有观察到分析剂度的临床显著变化.
结论:
- 板式离心法是一种有前途的方法,可以减少脂血,同时保持可靠的结果.
- 需要进一步研究更大的样本大小和温度分析.
- 这种技术提供了潜在的实验室实用性,平衡实用性和准确性.
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