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Updated: Jun 4, 2025

Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy 3D-SIM
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Measuring Properties of the Membrane Periodic Skeleton of the Axon Initial Segment using 3D-Structured Illumination Microscopy 3D-SIM

Published on: February 11, 2022

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在同位素100nm分辨率下用4Pi-SIM阐明亚细胞结构和动态

Zijing Ouyang1,2, Qian Wang3,4,5, Xiaoyu Li6

  • 1Biomedical Engineering Department, Institute of Advanced Clinical Medicine, State Key Laboratory of Molecular Oncology, International Cancer Institute, Peking University, Beijing, China.

Nature methods
|December 23, 2024
PubMed
概括

介绍 4Pi-SIM,这是一种新的显微镜技术,它结合了 3D 结构化照明显微镜 (3D-SIM) 和干涉度方法,以实现同位素超分辨率. 这一突破使得亚细胞结构和动态的高保真纳米级成像成为可能.

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The cytoskeletal architecture can be studied using different microscopic and biochemical techniques. Electron microscopy was instrumental in discovering the cytoskeletal architecture around the 1960s, which allowed obtaining structural information at a high-resolution level. However, the sample preparation procedure often limits this ability in biological samples. Several protocols have been developed over the years to optimize sample preparation. In one of the protocols known as rotary...
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