相关实验视频

Updated: Jun 4, 2025

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Glycomics-Guided Glycoproteomics Facilitates Comprehensive Profiling of the Glycoproteome in Complex Tumor Microenvironments

Published on: February 7, 2025

基于质谱的糖组和蛋白组分析,从复杂的生物样本中对片上消化的组织进行剖析

Sayantani Chatterjee¹, Joseph Zaia^1,2, Manveen K Sethi³

¹Department of Biochemistry, Center for Biomedical Mass Spectrometry, Boston University School of Medicine, Boston, MA, USA.

Methods in molecular biology (Clifton, N.J.)

|December 23, 2024

概括

本研究介绍了一种在幻灯片上的组织消化方法,用于全面绘制葡萄糖蛋白质组图. 这种技术可以从小组织样本中详细分析糖氨基氨基甘,N结合甘和 (糖) .

科学领域:

生物化学生物化学
蛋白质组学是指蛋白质组学.
格莱科米克斯 (Glycomics) 是一个有趣的药物.

背景情况:

质谱学对于分析复杂的生物样本至关重要.
目前用于组织糖蛋白质组分析的方法可能受到样本变异性和区域特异性的限制.

研究的目的:

从小组织样本开发一种简单,具有成本效益的方法,用于从小组织样本进行全面的葡萄糖蛋白质组映射.
为了能够同时分析糖氨基氨基甘氨酸,N连接甘氨酸和 (糖) .

主要方法:

对于新鲜冷或固定在甲中的氨酸嵌入式组织幻灯片的幻灯片上组织消化协议.
序列的酶消化和脱盐,没有先前的衍生.
液体染色学-双重质谱仪用于生物分子的识别和量化.

主要成果:

同时识别糖氨基甘氨酸脱糖体 (hyaluronan, chondroitin 硫酸盐, heparan 硫酸盐), N 链 glycans 和完整的 (glyco) .
从单个组织斑点详细描述了分糖化合物组成,甘氨酸结构,丰度和特定位点的甘氨酸占用率.
适用于小于10nL的组织体积的方法.

结论:

幻灯片消化方法提供了从最小的组织样本中进行深入的葡萄糖蛋白质组分析.
这种方法为生物医学实验室提供了一个强大的和可传播的工具.
能够详细描述组织组成和分子动态.

关键词:

固定在形式氨酸中的氨酸嵌入式组织.新鲜的冷组织.格莱科米克斯 (Glycomics) 是一个有趣的药物.葡萄糖蛋白质组学液体色谱学液体色谱学液体色谱学质谱测量质量谱测量在幻灯片上进行组织消化.蛋白质组学是指蛋白质组学.

更多相关视频

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Mass Spectrometric Analysis of Glycosphingolipid Antigens

Published on: April 16, 2013

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Sample Preparation for Metabolic Profiling using MALDI Mass Spectrometry Imaging

Published on: December 22, 2020

相关概念视频

MALDI-TOF Mass Spectrometry

MALDI-TOF Mass Spectrometry

Mass spectrometry is a powerful characterization technique that can identify and separate a wide variety of compounds ranging from chemical to biological entities, based on their mass-to-charge ratio (m/z). The instruments that allow this detection, known as mass spectrometers, have three components: an ion source, a mass analyzer, and a detector. These spectrometers differ based on the nature of their ion source and analyzers.
Matrix-assisted laser desorption ionization (MALDI) is a commonly...

Mass Spectrometry: Complex Analysis

Mass Spectrometry: Complex Analysis

Mass spectrometry is an important technique for the identification of pure compounds. However, it has some limitations for the analysis of complex mixtures, often due to excessive fragmentation making the spectrum too complicated to decipher. Mass spectrometry can be combined with suitable separation methods in sequence, forming hyphenated methods, which are useful in the analysis of complex mixtures.
GC–MS is a powerful hyphenated method commonly used in forensics and environmental...

Peptide Identification Using Tandem Mass Spectrometry

Peptide Identification Using Tandem Mass Spectrometry

Tandem mass spectrometry, also known as MS/MS or MS2, is an analytical technique that employs two mass analyzers. Essentially it is a series of mass spectrometers that helps isolate a particular biomolecule and then helps study its chemical properties.
This technique helps gather information regarding the protein from which the peptide was obtained and to study the peptides’ amino acid sequence. Identifying peptides from a complex mixture is an important component of the growing field of...