相关实验视频

Updated: Jun 4, 2025

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Generation of hiPSC-Derived Intestinal Organoids for Developmental and Disease Modelling Applications

Published on: March 8, 2024

人体结肠有机体与诱导的多能干细胞的分化,使用一种改进的方法

I-Ting Lee¹, Yu Takahashi¹, Takashi Sasaki¹

¹Food Biochemistry Laboratory, Department of Applied Biological Chemistry, Graduate School of Agricultural and Life Sciences, The University of Tokyo, Japan.

|December 23, 2024

概括

这项研究完善了人类结肠有机体 (HCO) 与诱导多能干细胞 (iPSC) 的分化. 优化的协议增强结肠特异性标记物和成熟,以更好地建模疾病.

科学领域:

干细胞生物学干细胞生物学
胃肠道生理学胃肠道生理学
机器人技术是机器人技术.

背景情况:

结肠表皮对于胃肠道平衡至关重要.
人体结肠有机体 (HCOs) 是研究结肠生理学的宝贵模型.
目前用于将诱导多能干细胞 (iPSC) 分化为HCOs的方法缺乏标准化,限制了它们的使用.

研究的目的:

优化分化协议,从iPSCs生成成熟的人体结肠器官 (HCOs).
将现有协议进行比较,并确定增强结肠身份和成熟的关键因素.
提高HCOs作为结肠研究模型的可靠性.

主要方法:

对两个已建立的iPSC与HCO差异化协议进行比较分析.
使用过渡性骨形态基因蛋白2 (BMP2) 信号激活.
使用腺病毒介导的HOXD13或SATB2转录因子转导.

主要成果:

涉及过渡性BMP2激活的协议显示,结肠特异性标记物的优异调节.
与HOXD13或SATB2转导同时使用BMP2显著增强了结肠识别.
优化的协议导致了更成熟的HCOs.

结论:

精细的分化协议显著改善了成熟的人类结肠器官的产生.
增强的HCO为研究结肠上皮细胞生物学和病理学提供了更强大的模型.
这一进步有助于进一步研究胃肠道疾病.

关键词:

在 BMP2 信号传输中.这就是HOXD13D.在SATB2中,SATB2是SATB2的第二种类型.结肠有机体有机体诱导多能干细胞的诱导干细胞.

更多相关视频

Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

Using Human Induced Pluripotent Stem Cell-derived Intestinal Organoids to Study and Modify Epithelial Cell Protection Against Salmonella and Other Pathogens

Published on: May 12, 2019

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Establishment of Human Epithelial Enteroids and Colonoids from Whole Tissue and Biopsy

Published on: March 6, 2015

相关概念视频

iPS Cell Differentiation

iPS Cell Differentiation

The ability of induced pluripotent stem cells or iPSCs to differentiate into most body cell types has stimulated repair and regenerative medicine research over the past few decades. iPSC-derived blood cells, hepatocytes, beta islet cells, cardiomyocytes, neurons, and other cell types can repair injuries or regenerate damaged tissue in diseases such as diabetes and neurodegenerative disorders.