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在Cupriavidus necator H16中以H₂驱动的西利生产

Tytti Jämsä¹, Nico J Claassens², Laura Salusjärvi³

¹VTT Technical Research Centre of Finland Ltd., Tekniikantie 21, 02150, Espoo, Finland. tytti.jamsa@vtt.fi.

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|December 24, 2024

概括

可再生能为生物催化剂提供动力,用于使用改造的Cupriavidus necator生产西利. 这种绿色化学方法有效地回收合作因子,以实现可持续的生物转化.

科学领域:

生物技术是生物技术.
绿色化学绿色化学
代谢工程是代谢工程.

背景情况:

生物催化剂为传统化学合成提供了一个可持续的替代方案.
作为一个高效和可再生的减少剂,用于生物催化过程中的辅因子再生.
用NAD依赖酶进行工程的Cupriavidus necator H16促进了辅因子回收.

研究的目的:

开发一种生物催化系统,用于用用于辅助因子再生的D-西洛斯转化为西利.
为了在C. necator. 中异质地表达来自Scheffersomyces stipitis的D-克西洛斯减少酶.
为了评估在工程微生物宿主中以驱动的辅因子回收利用的效率.

主要方法:

在Cupriavidus necator中,D-糖还原酶的异质表达.
使用静止细胞和作为还原剂,将D-西洛斯生物转化为西利.
对辅助因素回收效率和产品产量的分析.

主要成果:

通过成功地表达D-西洛斯减少酶,使得30g/L的D-西洛斯几乎完全转化为西利.
超过90%的源能量和质子被用于生物转化,这表明系统效率很高.
工程设计的C. necator底盘还从各自的基质中产生L-arabitol和D-ribitol,达到0.7g/L/h的最大氧化醇生产率.

结论:

可再生是一种有效的减少剂,用于驱动生物催化剂中的辅因子回收利用.
像C. necator这样的氧化细菌是驱动生物催化应用的合适宿主.
这项研究表明了可持续生产糖醇的有希望的途径.

关键词:

这就是Cupriavidus necator.拉尔斯托尼亚 (Ralstonia eutropha) 是一种缩的植物.生物转化生物转化辅助因子回收利用的回收利用复合因子再生的复合因子氧化细菌是一种氧化的细菌.

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