相关实验视频

Updated: May 8, 2025

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

A Cryo-pulverization Protocol for Processing Mouse Paws to Evaluate Molecular Pathways of Tissue Inflammation in a Collagen Induced Arthritis Model

Published on: October 30, 2019

综合性代谢-蛋白质组分析揭示了针对类风湿性关节炎的新疗法方法

Prachi Agnihotri^1,2, Mohd Saquib^1,2, Lovely Joshi^1,2

¹Integrative and Functional Biology Department, Council of Scientific & Industrial Research (CSIR)-Institute of Genomics and Integrative Biology, Mall Road, Delhi University Campus, Delhi, 110007, India.

Arthritis research & therapy

|December 24, 2024

概括

甘氨酸 (Glycoursodeoxycholic acid,GUDCA) 在类风湿性关节炎 (RA) 模型中显示出抗炎和抗氧化作用. 这种代谢物可能通过减少炎症和细胞增殖来提供一种新的治疗策略来管理RA.

科学领域:

生物化学生物化学
免疫学免疫学免疫学
药理学药理学是指药理学的学科.

背景情况:

类风湿性关节炎 (RA) 是一种慢性炎症疾病,治疗选择有限.
及时诊断和有效治疗RA仍然是一个重大挑战.
先进的奥米克学方法对于理解RA的发病过程至关重要.

研究的目的:

在类风湿性关节炎 (RA) 病变发生过程中识别独特的代谢物-蛋白质特征.
探索RA中已识别的代谢物的治疗潜力.
为了研究甘油酸 (Glycoursodeoxycholic acid) (GUDCA) 在RA中的作用.

主要方法:

在RA患者血上的非向代谢和蛋白质组.
在代谢学方面使用HPLC/LC-MS/MS,在蛋白质学方面使用SWATH.
在体外对TNF-α诱导细胞的研究以及在CIA大鼠模型中的体内研究.

主要成果:

确定了82种代谢物和231种不同的蛋白质.
发现了氨酸和叶绿素通路的显著变化,特别是甘油氧化醇酸 (GUDCA).
在实验室中,GUDCA表现出与IGF1和TTR蛋白相关的抗炎,抗增殖和ROS清除作用.

结论:

在低剂量时,GUDCA表现出强大的抗氧化和抗炎性质.
瓜德卡可以作为一种潜在的治疗药物治疗RA.
进一步研究GUDCA在RA中的机制和疗效是有必要的.

关键词:

炎症通路,CIA-Rat模型中的一个.代谢物的代谢物.代谢学代谢学代谢学蛋白质组学是指蛋白质组学.类风湿性关节炎 (RA) 是一种斯瓦特 (SWATH) 是一个流浪汉.

更多相关视频

Author Spotlight: Enhancing Rheumatoid Arthritis Research Through HR-pQCT Imaging Analysis

Author Spotlight: Enhancing Rheumatoid Arthritis Research Through HR-pQCT Imaging Analysis

Published on: October 6, 2023

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Co-immunoprecipitation Assay for Studying Functional Interactions Between Receptors and Enzymes

Published on: September 28, 2018

相关概念视频

Genome-wide Association Studies-GWAS

Genome-wide Association Studies-GWAS

Genome-wide association studies or GWAS are used to identify whether common SNPs are associated with certain diseases. Suppose specific SNPs are more frequently observed in individuals with a particular disease than those without the disease. In that case, those SNPs are said to be associated with the disease. Chi-square analysis is performed to check the probability of the allele likely to be associated with the disease.
GWAS does not require the identification of the target gene involved in...

The JAK-STAT Signaling Pathway

The JAK-STAT Signaling Pathway

Several cytokine receptors have tightly bound Janus kinase or JAK proteins attached at their cytosolic tail. Small signaling molecules such as cytokines, growth hormones, or prolactins bind to the cytokine receptors and initiate their dimerization. The dimerization brings the cytosolic JAKs together that trans-phosphorylate and activates each other. The activated JAKs now phosphorylate cytosolic tails of the cytokine receptors, which serve as binding sites for adaptor proteins such as SH2...

Proteomics

Proteomics

A proteome is the entire set of proteins that a cell type produces. We can study proteomes using the knowledge of genomes because genes code for mRNAs, and the mRNAs encode proteins. Although mRNA analysis is a step in the right direction, not all mRNAs are translated into proteins.
Proteomics is the study of proteomes' function. It involves the large-scale systematic study of the proteome to denote the protein complement expressed by a genome. Scientist Mark Wilkins coined the term...