腺病毒感染的HERV重新激活与病毒免疫调节有关
Wen Liang1, Miona Stubbe2, Lisa Pleninger1
1Institute of Virology, School of Medicine, Technical University of Munich, Munich, Germany; Institute of Virology, Helmholtz Zentrum München, Munich, Germany.
人类腺病毒C血清型5 (HAdV-C5) 感染在肝细胞中重新激活人体内源逆转录病毒 (HERVs). 这种重新激活会影响参与病毒致癌和免疫反应的基因.
科学领域:
- 病毒学 病毒学
- 基因组学就是基因组学.
- 免疫学 免疫学 免疫学
背景情况:
- 人体内源逆转录病毒 (HERVs) 通常被静止,但可以被病毒感染等环境因素重新激活.
- 了解 HERV 反激活对于理解病毒病原和宿主免疫反应至关重要.
研究的目的:
- 在感染人类腺病毒C血清型5 (HAdV-C5) 后,研究HERVs在人类肝细胞 (HepaRG) 中的转录活性.
- 为了确定重新激活的HERVs在与病毒致癌和炎症相关的基因表达变化中的潜在作用.
主要方法:
- 肝炎RG细胞感染HAdV-C5.5病毒
- 对HERV转录活性和差异性基因表达的分析.
- 对上调HERVs和基因进行染色体近距离分析.
主要成果:
- HAdV-C5感染导致了几组HERV的重新激活,并在HepaRG细胞中改变了基因表达.
- 在激活HERV位点附近的上调基因与病毒致癌和炎症信号通路有关.
- 确定了一种含有ERVK9-11序列的FBXO17转录,这表明与干扰素反应调节的联系.
结论:
- HAdV-C5感染可以诱导HERV的重新激活,可能会影响宿主基因网络.
- 再激活的HERVs可能在调节免疫反应,特别是干扰素通路方面发挥作用.
- 特定的HERV群体可能参与触发与病毒病原和炎症相关的基因网络.
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