分散方法不影响多壁碳纳米管的体外基因毒性,尽管诱导了表面变化
Michael J Burgum1, Víctor Alcolea-Rodríguez2, Hanna Saarelainen3
1In Vitro Toxicology Group, Faculty of Medicine, Health and Life Sciences, Institute of Life Sciences, Swansea University Medical School, Singleton Park, Swansea SA2 8PP, UK.
NanoImpact
|December 24, 2024
概括
多壁碳纳米管 (MWCNTs) 显示在人类细胞中没有遗传毒性,尽管有一些氧化潜力. 进一步的研究应该集中在固定DNA损伤上,以解释细胞修复机制.
科学领域:
- 纳米材料的安全性
- 毒理学 毒理学 毒理学
- 基因毒性测试是指基因毒性测试.
背景情况:
- 多壁碳纳米管 (MWCNTs) 是具有广泛应用的高比例纳米材料 (HARNs).
- 增加的职业和消费者暴露需要对潜在的人类健康危害进行调查.
- 了解MWCNT的基因毒性对于风险评估至关重要.
研究的目的:
- 研究NM-400和NM-401 MWCNTs在人类淋巴细胞 (TK6) 和肺上皮细胞 (16HBE14o-) 中的基因毒性.
- 用共同培养模型评估二次基因毒性.
- 评估不同分散方法对MWCNT特性和潜在毒性的影响.
主要方法:
- 实验室哺乳动物细胞基因突变和细胞动力阻断微核 (CBMN) 试验被采用.
- 在16HBE14o和dTHP-1细胞的单培养和共同培养上进行CBMN测定.
- 进行了MWCNT分散方法 (纳米GenoTox与高剪切混合) 的比较,通过电子显微镜,拉曼光谱和化学分析进行了表征.
主要成果:
- 在分散后观察到NM-400 MWCNTs的结构损伤,在探头声波检测中观察到更大的氧化转变.
- 在测试条件下,在TK6或16HBE14o-细胞中没有检测到统计学上显著的基因毒性.
- 观察到MWCNTs的最小细胞内化,可能与负面的基因毒性发现相关.
结论:
- 尽管表现出氧化潜力,但NM-400和NM-401 MWCNTs在测试的人类细胞系中没有诱导遗传毒性.
- 缺乏观察到的基因毒性可能归因于细胞吸收有限和高效的DNA修复机制.
- 未来的研究应该优先考虑测量固定DNA损伤的终点,以充分评估纳米材料的生物影响.
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