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对于与人类骨髓病变和疼痛相关的组织病理学的鼠标骨关节炎骨评分

Daniel F McWilliams^1,2, Mohsen Shahtaheri^1,2, Soraya Koushesh³

¹Pain Centre Versus Arthritis and Academic Unit of Injury, Recovery and Inflammation Sciences, University of Nottingham, UK.

Osteoarthritis and cartilage open

|December 24, 2024

概括

一个新的老鼠关节骨炎 (OA) 评分系统,老鼠关节骨炎骨评分 (rOABS),可靠地测量了亚冠状腺骨病理. 这种评分系统与OA老鼠模型中的结构损伤和疼痛行为相关.

科学领域:

整形外科整形外科整形外科
类风湿病学类风湿病学
翻译医学是一种翻译医学.

背景情况:

人类膝关节骨关节炎 (OA) 涉及骨髓病变 (BMLs) 等子冠状腺骨病理,包括炎症,纤维化和血管变化.
在临床前模型中了解和量化这些子冠状腺变化对于开发有效的OA治疗非常重要.

研究的目的:

开发和验证膝关节骨关节炎 (OA) 的大鼠模型中的组织学评分方法,可与人类的BMLs相比较.
评估经过修改的OA骨评分 (rOABS) 与OA结构变化和大鼠疼痛行为的可靠性和相关性.

主要方法:

两种大鼠OA膝关节疼痛模型被诱导使用单离子酸盐 (MIA) 注射或阴茎切割 (MNX).
基因组学膝盖部分被评分为软骨病变,骨质细胞,突炎,并使用修改后的老鼠骨关节炎骨评分 (rOABS).
测量了疼痛行为,包括承重不对称性和后腿撤回值. 通过使用类内相关系数 (ICC) 评估rOABS的评价者间可靠性.

主要成果:

rOABS表现出良好的评价者间可靠性 (ICC = 0.79),并且在两个OA模型中与对照组相比显著更高 (p < 0.003).
rOABS评分与软骨损伤 (rho = 0.69),骨菌形成 (rho = 0.61) 和突炎 (rho = 0.76) 有着强烈的关联.
较高的ROABS分数与增加的疼痛行为有显著的相关性,包括承重不对称 (rho = 0.65) 和降低的脚撤回值 (rho = -0.47).

结论:

开发的老鼠骨关节炎骨评分 (rOABS) 可靠地量化了老鼠OA模型中的子冠状腺骨病理.
rOABS有效地捕捉了与人类BML相比的关键病理特征.
这种经过验证的评分系统为在临床前研究中评估骨关节炎的进展和治疗疗效提供了有价值的工具,将结构性病理与疼痛结果联系起来.

关键词:

骨髓病变 - 骨髓病变 - 骨髓病变历史学历史学历史学进行阴茎切除术 (meniscectomy).单离子乙酸盐的使用方法骨关节炎是一种骨关节炎.鼠标鼠标鼠标鼠标

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