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在产前症期间,NETs通过高流动性组蛋白B1加剧了胎盘炎症和损伤

Yurong Lu¹, Yijia Tian², Xiao Liu³

¹Department of Obstetrics, The Affiliated Taian City Central Hospital of Qingdao University, Taian, 271000, China; Department of Obstetrics and Gynecology, Shandong Provincial Hospital Affiliated to Shandong First Medical University, Jinan, Shandong, 250021, China; Cheeloo College of Medicine, Shandong University, Jinan, Shandong, 250012, China.

|December 24, 2024

概括

中性粒细胞外细胞陷 (NETs) 通过通过高流动性组盒1 (HMGB1) 损害热囊细胞,从而导致妊娠前 (PE). 针对HMGB1可能通过减少NETs引起的损害,为PE提供未来的治疗方法.

科学领域:

生殖免疫学生殖免疫学
怀孕的病理生理学怀孕的病理生理学

背景情况:

孕前 (PE) 涉及在母胎界面的炎症性压力.
病理性中性粒细胞外陷 (NETs) 涉及PE病变,但机制尚不清楚.

研究的目的:

为了研究高流动性组框1 (HMGB1) 在预先怀孕症发病过程中在NET中发挥的作用.
阐明NETs对PE中热囊细胞功能障碍的贡献机制.

主要方法:

使用ELISA对PE患者血中的量化NET.
在PE患者中通过西部斑块和PCR评估胎盘HMGB1表达.
在体外研究中,人体热囊细胞 (HTR-8/SVneo) 暴露在NET中,以评估增殖,入侵,迁移,亡,氧化应激和炎症因子分泌.

主要成果:

在PE患者的外周血液中发现了升高的NETs水平.
在PE胎盘中观察到HMGB1表达的增加,与NETs同定位.
NETs 损害了 trofhoblast 细胞的入侵和迁移,增加了氧化应激 (ROS) 和升高的炎症因子 (IL-1β,IL-6,IL-8,TNF-α),这些影响可以通过 HMGB1 清除剂 glycyrrhizin 回转.

结论:

在孕前症中,NETs通过HMGB1诱导热囊细胞炎症和功能损伤.
在PE中,HMGB1是NETs驱动的氧化应激级联中的关键调解者.
抑制HMGB1以减轻NETs诱导的损伤是PE的潜在治疗策略.

关键词:

这就是HMGB1的特点.网络网络网络网络中性粒细胞细胞外陷孕前症孕前症热囊细胞细胞的细胞.

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