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在体外基于便细菌和益生菌进行的daidzin生物转化分析

Yuqing Wang¹, Zhe Li¹, Dongxue Wu¹

¹Guang'an Men Hospital, China Academy of Chinese Medical Sciences, Beijing 100010, China; School of Pharmacy, Shandong University of Traditional Chinese Medicine, Jinan 250355, China.

Journal of pharmaceutical and biomedical analysis

|December 24, 2024

概括

这项研究研究了肠道细菌对大素的代谢,确定了31种代谢物和肠道菌群的显著变化,特别是Bifidobacterium. 这些发现揭示了大的生物转化途径和对肠道微生物群的影响.

科学领域:

微生物学和生物化学
药理学和毒理学药理学和毒理学
肠道微生物组研究研究

背景情况:

戴津是一种异黄糖化物,具有各种生物活性,但吸收能力有限.
肠道菌群对大津的代谢途径在很大程度上没有表征,这阻碍了它的应用.
了解大津的肠道微生物代谢对于其治疗潜力至关重要.

研究的目的:

通过肠道菌群来阐明戴津的代谢命运.
为了识别和量化daidzin代谢物.
调查大津对肠道微生物组成和功能的影响.

主要方法:

高性能液态染色学 (HPLC) 和超高性能液态染色学结合高分辨率质谱学 (UHPLC-HRMS) 进行代谢物分析.
16S rRNA测序以评估肠道菌群组成的变化.
在体外发酵系统使用便细菌和精选的益生菌.

主要成果:

戴兹显著下降,而其聚糖体,戴兹,增加,表明生物转化.
确定了31种代谢物,这些代谢物来自化,甲基化和环裂变等过程.
戴津改变了肠道微生物群的组成,显著增加了Bifidobacterium的丰富性 (p < 0.05).
益生菌发酵系统证实了已识别的津转化途径.

结论:

肠道菌群广泛地将大津代谢成各种化合物.
戴津影响肠道微生物社区结构,促进像Bifidobacterium这样的有益细菌.
这项研究为大津便代谢物分析建立了一个新型模型,为进一步的研究铺平了道路.

关键词:

戴德津就是这样一个人.便细菌便细菌是一种它的代谢物是代谢物.益生菌益生菌益生菌在UHPLC-HRMS中使用.

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