хлорпирифос通过破坏甲状腺激素信号通路来影响子的发育
Aoxue Wang1, Yuanyuan Liu1, Yujia Yan1
1Institute of Pesticide and Environmental Toxicology, College of Agriculture and Biotechnology, Zhejiang University, Hangzhou 310058, P. R. China.
Environmental science & technology
|December 24, 2024
概括
хлорпирифос (CPF) 通过干扰甲状腺激素信号来破坏两动物的变形. 这种杀虫剂作为甲状腺激素受体α (TRα) 的部分激动剂,在环境相关的度下影响发育.
科学领域:
- 环境毒理学环境毒理学
- 发展生物学 发展生物学
- 内分泌学 在内分泌学.
背景情况:
- 类杀虫剂 (CPF) 等有机类杀虫剂对水生生态系统构成风险.
- 虽然CPF的神经毒性已知,但其对两变形和甲状腺激素受体相互作用的影响需要进一步调查.
研究的目的:
- 为了研究CPF对*Xenopus laevis*变形的特定阶段的影响.
- 阐明CPF与甲状腺激素受体α (TRα) 的分子相互作用.
主要方法:
- 暴露于环境相关的CPF度 (1.8和18μg/L) 的Xenopus laevis.
- 对变形进展,甲状腺激素敏感基因表达和T3/T4水平的分析.
- 分子对接,表面等离子体共振 (SPR) 和 luciferase 记者基因测试来评估 TRα 结合和活性.
主要成果:
- 暴露于CPF改变了前变态进展和TH-响应基因表达.
- 在18μg/L CPF时,观察到三甲状腺素 (T3) 和甲状腺素 (T4) 水平的显著降低.
- CPF证明了TRα与T3的竞争性结合,并充当了部分激动剂,调节受体活性.
结论:
- CPF以阶段依赖的方式破坏两动物甲状腺信号传输.
- CPF对TRα的部分激动作用有助于对两动物发育产生不良影响.
- 研究结果强调CPF是影响水生脊椎动物的内分泌干扰物.
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