相关实验视频

Updated: Jun 4, 2025

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Using Coculture to Detect Chemically Mediated Interspecies Interactions

Published on: October 31, 2013

释放绿色生物制造潜力:在Bacillus subtilis中使用T7集成过度表达系统的优异基因表达

Zhi-Yuan Yao^1,2, Min-Jun Yu¹, Qu-Quan Li³

¹Key Laboratory of Carbohydrate Chemistry and Biotechnology of Ministry of Education, School of Life Sciences and Health Engineering, Jiangnan University, Wuxi 214122, PR China.

ACS synthetic biology

|December 24, 2024

概括

研究人员开发了一个新的基因表达系统,用于 Bacillus subtilis,增强生物制造. 这种无诱导器的系统可以提高生产有价值的工业产品的控制和效率.

科学领域:

工业生物技术工业生物技术
合成生物学合成生物学
微生物工程是微生物的工程.

背景情况:

细菌是酶和营养产品可持续生物生产的关键宿主.
现有的基因表达系统通常需要抗生素或诱导剂,限制效率和可持续性.
开发可控制和高效的表达系统对于推进工业生物技术至关重要.

研究的目的:

在Bacillus subtilis中建立一个双模块T7整合表达系统.
调查基因组整合部位对基因表达强度的影响.
为了实现高水平的,无诱导体的基因表达,用于生物制造.

主要方法:

在B. subtilis基因组中整合T7RNA聚合酶的CRISPR/Cas9系统.
一个表达控制模块系统地集成到28个染色体位置.
修改LacI抑制器以实现无诱导生物调节.
对表达脂酶D和 hialuronic acid lyase 的系统的应用.

主要成果:

一个高强度的综合基因表达系统是通过优化染色体位置来开发的.
通过 LacI 抑制剂修饰,在没有诱导剂添加的情况下实现了强烈的基因表达.
细胞外酶活性达到了339.12U/mL的脂酶D和2.60×10^4U/mL的氨酸酶.
在5L发酵尺度下,达到6.86g/L的氨酸生产产量.

结论:

开发的系统为B. subtilis提供了可控制,高效和无诱导剂的基因表达工具.
这种系统显著提高了B. subtilis在生物制造应用中的潜力.
消除抗生素和诱导剂促进了更可持续和更具成本效益的生物生产过程.

关键词:

这种细菌是 Bacillus subtilis.生物合成途径的调节染色体整合部位是染色体的整合部位.整合表达系统整合表达系统再组合蛋白的表达方式.

更多相关视频

The MultiBac Protein Complex Production Platform at the EMBL

The MultiBac Protein Complex Production Platform at the EMBL

Published on: July 11, 2013

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Inducible T7 RNA Polymerase-mediated Multigene Expression System, pMGX

Published on: June 27, 2017

相关概念视频

Transgenic Plants

Transgenic Plants

Recombinant DNA technology called transgenesis is often used to add a foreign gene or remove a detrimental gene from an organism. Such genetically modified organisms are called transgenic organisms.
The first-ever transgenic plant was a tobacco plant developed in 1983 that showed resistance against the tobacco mosaic virus. Since then, many transgenic plants have been developed and commercialized for improving the agricultural, ornamental, and horticultural value of a crop plant. Transgenic...

Transgenic Organisms

Transgenic Organisms

Reporter Genes

Reporter Genes

Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.

Bacterial Transformation

Bacterial Transformation

In 1928, bacteriologist Frederick Griffith worked on a vaccine for pneumonia, which is caused by Streptococcus pneumoniae bacteria. Griffith studied two pneumonia strains in mice: one pathogenic and one non-pathogenic. Only the pathogenic strain killed host mice.
Griffith made an unexpected discovery when he killed the pathogenic strain and mixed its remains with the live, non-pathogenic strain. Not only did the mixture kill host mice, but it also contained living pathogenic bacteria that...