将废木材转化为纯L-(+) -乳酸:通过细胞循环连续发酵有效利用混合糖介质
Linda Schroedter1, Roland Schneider1, Joachim Venus1
1Leibniz Institute for Agricultural Engineering and Bioeconomy e. V. (ATB), Department Microbiome Biotechnology, Max-Eyth-Allee 100, Potsdam 14469, Germany.
Bioresource technology
|December 24, 2024
概括
这项研究利用热友细菌优化了从纤维素素酸中生产的乳酸. 细胞循环连续发酵通过克服糖利用限制,显著提高了生产力.
科学领域:
- 生物技术是生物技术.
- 工业微生物学 工业微生物学
- 生物化学工程 生物化学工程
背景情况:
- 红细胞质介质由于糖和抑制剂而存在挑战.
- 从废物生物质中有效生产L-(+) -乳酸至关重要.
研究的目的:
- 评估热敏的海因德里克西亚凝固剂,用于从废木水解盐中产生乳酸.
- 为了克服批量发酵的局限性,使用连续的细胞循环过程.
主要方法:
- 使用H.凝固素的批量发酵研究 A166.6.
- 细胞循环连续发酵,优化初始稀释速率.
- 在合成介质上进行长时间的过程监控.
主要成果:
- 凝血剂A166在批量模式下达到高乳酸标位 (94.6 g/L),耐受抑制剂.
- 碳催化剂抑制在批量模式下限制了生产力.
- 连续发酵达到7.6g/L/h的生产率,比批量增加4.5-5.8倍,糖的利用完全.
- 在合成介质上实现了长达188小时的延长过程持续时间.
结论:
- 细胞循环连续发酵克服了基于纤维素蛋白的乳酸生产的局限性.
- 优化的稀释速率和耐受抑制剂的菌株,如H.coagulans A166是有效生物处理的关键.
- 这种方法显示出从纤维素酸原料中工业规模的乳酸回收显著的前景.
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