用于组织工程的肝素化原基水凝:物理,机械和生物特性
Meiling Wu1, Anne Sapin-Minet1, Loïc Stefan2
1Université de Lorraine, CITHEFOR, F-54000 Nancy, France.
International journal of pharmaceutics
|December 24, 2024
概括
肝素化原蛋白水凝被开发用于血管移植,显示出更好的血液兼容性和抗血栓性质. 这些生物相容的涂层有效地保留了七天以上的肝素,增强了血液接触材料的潜力.
科学领域:
- 生物材料科学 生物材料科学
- 血管工程 血管工程
- 生物化学 生物化学
背景情况:
- 原蛋白是一种主要的血管细胞外矩阵蛋白质,具有较弱的抗原性,因此适用于血管移植涂层.
- 肝素化是一种优选的方法,可以赋予原蛋白抗血栓性质,改善血液兼容性,并使生物活性分子的输送成为可能.
研究的目的:
- 使用pH驱动的自组装制造基基基凝.
- 为了评估这些水凝的物理化学和生物相容性,用于血管移植应用.
- 评估开发的涂层的抗血栓疗效和长期肝素保留.
主要方法:
- 以pH为驱动的自我组装,以形成以原为基础的水凝,并加入低分子量肝素 (LMWH) 或未分离的肝素 (UFH).
- 物理化学表征:凝动力学,扩散,粘性弹性,微观结构和肝素量化.
- 生物相容性评估:细胞相容性,白蛋白吸附和细胞活力.
- 抗血栓评估:肝素释放,激活的部分血栓形成时间 (aPTT),血栓时间 (TT) 和血栓生成试验.
主要成果:
- 与低分子量肝素 (20%) 相比,未分离肝素 (60-80%) 的承载能力更高.
- 增加的肝素度 (6 IU/mL) 导致更柔软的水凝具有增强的扩散性和改善的抗血栓活性 (延迟凝血,取消了血栓生成).
- 水凝呈现出分层,网络结构,并保持至少七天的肝素释放,证明生物相容性与低色素吸附,对细胞活力没有影响.
结论:
- 成功开发出具有可调整的物理化学特性和显著的抗血栓能力的肝素化原蛋白水凝.
- 这些水凝显示出出色的生物相容性和长期的肝素保留,适用于先进的血液接触材料.
- 这些发现突显了这些涂层在开发高效安全的血管移植方面的潜力.
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