相关实验视频

Updated: Jul 20, 2026

High-throughput Saccharification Assay for Lignocellulosic Materials

High-throughput Saccharification Assay for Lignocellulosic Materials

Published on: July 3, 2011

准备,表征和活动验证从大草草的糖

Wenming Jiang^1,2,3, Fang Li²

¹School of Environmental and Quality Testing, Chongqing Chemical Industry Vocational College, Chongqing, 401228, China.

|December 25, 2024

概括

草的废弃物被用xylanase转化为有价值的二氧化二氧化糖 (XOS). 这些XOS表现出显著的抗氧化和益生菌特性,增强了它们的利用潜力.

科学领域:

生物质的价值化生物质的价值化
食品科学食品科学食品科学
生物化学生化学

背景情况:

草的利用价值很低. 草的使用价值很低.
氧氧糖 (Xylooligosaccharides,XOS) 是一种具有潜在健康益处的功能性糖.
开发XOS生产的可持续方法至关重要.

研究的目的:

用于从香草草中产生氧化氧化糖 (XOS).
为了描述产生的XOS的物理化学特性.
评估XOS的抗氧化和益生菌活性.

主要方法:

植物的西兰酶水解以获得XOS.
对于蛋白质和核酸去除的Sevag方法.
高性能液态色谱 (HPLC) 用于糖成分分析.
扫描电子显微镜 (SEM),富里埃变换红外光谱 (FTIR) 和核磁共振 (NMR) 用于结构特征.
对抗氧化活性进行DPPH清除试验和降低功率试验.
针对特定益生菌细菌的抗菌活性测定.

主要成果:

通过西兰酶水解,成功地从香草中产生了XOS.
鉴定证实了各种糖的存在,其中西洛斯是最丰富的 (35.04%).
SEM显示了粗的,交叉连接的表面,FTIR显示了β-糖化键,而NMR证实了1-4个糖化键.
与半纤维素B相比,XOS表现出增强的抗氧化能力 (DPPH清理和减少功率).
在XOS的研究中,它对Lactobacillus acidophilus和Bifidobacterium animalis产生了有益的益生菌作用.

结论:

草是生产具有有价值的功能性质的XOS的可行来源.
生产的XOS具有显著的抗氧化剂和益生菌潜力.
这项研究强调了一种可持续的方法,即将农业废物转化为高价值的功能成分.

关键词:

具有抗氧化活性的抗氧化剂.植物草的草.结构性表征结构性表征糖酸酸盐制剂糖酸酸酸制剂

更多相关视频

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Sequencing of Plant Wall Heteroxylans Using Enzymic, Chemical Methylation and Physical Mass Spectrometry, Nuclear Magnetic Resonance Techniques

Published on: March 24, 2016

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Structural Characterization of Mannan Cell Wall Polysaccharides in Plants Using PACE

Published on: October 16, 2017

相关概念视频

Oligosaccharide Assembly

Oligosaccharide Assembly

Protein glycosylation starts in the ER lumen and continues in the Golgi apparatus. Glycosyltransferases catalyze the addition of sugar molecules or glycosylation of proteins. Usually, these enzymes add sugars to the hydroxyl groups of selected serine or threonine residues to form O-linked glycans or the amino groups of asparagine residues to form N-linked glycans. Different positions on the same polypeptide chain can contain differently linked glycans.
Multiple sugar molecules that may or may...

Biosynthesis of Polysaccharides

Biosynthesis of Polysaccharides

Polysaccharides such as glycogen and starch are synthesized from nucleoside diphosphate sugars, primarily uridine diphosphate glucose (UDPG) and adenosine diphosphate glucose (ADPG). These activated glucose donors act as key intermediates in carbohydrate metabolism and biosynthesis. UDPG primarily involves glycogen synthesis in animals and many bacteria, while ADPG plays a fundamental role in starch synthesis in plants and certain bacteria.UDPG is formed when glucose-1-phosphate reacts with...

Production of Alcohol

Production of Alcohol

Continuous fermentation is a key strategy in industrial ethanol production, particularly when efficiency, scalability, and high yields are essential. This approach allows for uninterrupted operation and optimized resource utilization. The primary feedstock, corn starch, undergoes enzymatic hydrolysis facilitated by α-amylase and glucoamylase. These enzymes break down the starch into fermentable sugars such as glucose, which are readily assimilated by fermentative microorganisms.Fermentation...

Production of Organic Acids

Production of Organic Acids

Lactic acid, an important organic acid extensively applied in food, pharmaceutical, and biodegradable polymer industries, is primarily produced via microbial fermentation. This method is favored over chemical synthesis due to its environmental sustainability and capacity for enantiomerically pure product formation. Among various microbial processes, the fermentation of starch-based substrates stands out due to the abundance and renewability of raw materials like corn and potatoes.Hydrolysis of...