相关实验视频

Updated: Jun 4, 2025

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

In Situ Detection and Single Cell Quantification of Metal Oxide Nanoparticles Using Nuclear Microprobe Analysis

Published on: February 3, 2018

在PIXE的干矩阵斑点中对生物样本进行直接的多元件分析

Matea Krmpotić¹, Madina Telkhozhayeva², Merav Nadav Tsubery³

¹Division of Experimental Physics, Ruđer Bošković Institute, 10000, Zagreb, Croatia.

|December 25, 2024

概括

干矩阵点 (DMS) 采样使人体血液和血中的直接多元素分析能够使用粒子诱导X射线发射 (PIXE). 这种高效的方法需要最小的样本量,并提供精确的基本元素量化.

科学领域:

分析化学分析化学
生物医学分析医学分析
原子物理原子物理

背景情况:

干燥矩阵点 (DMS) 方法,最初用于新生儿查,越来越多地用于研究,以小样本体积的效率.
现有的生物样本分析技术往往需要更大的体积,涉及样本准备,或对矩阵效应敏感.

研究的目的:

系统地研究使用粒子诱导X射线发射 (PIXE) 的人类血液和血干燥矩阵斑点的直接多元件分析.
确定DMS-PIXE的适用性,用于量化生物样本中的基本元素,样本体积最小,不需要复杂的制备.

主要方法:

开发并验证了使用粒子诱导X射线发射 (PIXE) 的DMS直接多元元素分析方法.
采用内部标准补充来管理DMS异质性并实现单点测量.
用参考材料测试方法性能 (线性,准确性,精度,LOD) 并将其应用于人类血液和血样本.

主要成果:

在全血中量化Na,Mg,P,S,K,Ca,Fe和Zn,在血中量化Na,Mg,P,S,K和Ca,使用的样本仅为50μL.
与感应合等离子体 - 光学发射光谱学 (ICP-OES) 结果达成良好一致,表明高精度.
没有观察到显著的矩阵效应,证明了DMS-PIXE方法的稳定性.

结论:

粒子诱导X射线发射 (PIXE) 是生物样本中干矩阵斑点 (DMS) 的直接多元件分析的可行替代方案.
DMS-PIXE方法提供电解质和基本微量元素的灵敏检测 (mg/L水平),适合临床和研究应用.
这项研究证实了DMS采样是通过PIXE在血液和血中进行直接元素分析的实用方法.

关键词:

血液血液血液血液干燥的矩阵斑点干燥的矩阵斑点皮克斯 (PIXE) 是一个在等离子体中,我们可以得到等离子体.血清的血清是什么意思

更多相关视频

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Low Molecular Weight Protein Enrichment on Mesoporous Silica Thin Films for Biomarker Discovery

Published on: April 17, 2012

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Investigation of Microbial Cooperation via Imaging Mass Spectrometry Analysis of Bacterial Colonies Grown on Agar and in Tissue During Infection

Published on: November 18, 2022

相关概念视频

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-Assisted Laser Desorption Ionization (MALDI)

Matrix-assisted laser desorption ionization (MALDI) is a powerful analytical technique used in mass spectrometry. It enables the identification and characterization of various biomolecules, including proteins, peptides, nucleic acids, and carbohydrates. MALDI spectrometry is widely employed in biological and medical research, as well as in fields like pharmacology and biochemistry.
The analyte of interest, a biomolecule or a mixture of biomolecules, is mixed with a suitable matrix material. The...

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively Coupled Plasma Atomic Emission Spectroscopy: Instrumentation

Inductively coupled plasma (ICP) is the common plasma source used in atomic emission spectroscopy (AES), a technique that detects and analyzes various elements in a sample. This method is often called inductively coupled plasma atomic emission spectroscopy (ICP-AES).
There are three main types of inductively coupled plasma atomic emission spectroscopy (ICP-AES) instruments: sequential, simultaneous multichannel, and Fourier transform instruments, with the latter being less commonly used....

Atomic Emission Spectroscopy: Lab

Atomic Emission Spectroscopy: Lab

AES is a powerful analytical technique, especially effective when used with plasma sources, producing abundant spectra in characteristic emission lines. The Inductively Coupled Plasma (ICP), in particular, yields superior quantitative analytical data due to its high stability, low noise, low background, and minimal interferences under optimal experimental conditions. However, newer air-operated microwave sources are emerging as promising alternatives that could be more cost-effective than...