环境中的抗生素度诱导了Aeromonas hydrophila中的多素B耐受性
Yan Zhang1, Xinyuan Qiu1, Liwen Xu2
1Institute of Food Safety and Nutrition, Jinan University, Guangzhou 510632, China.
The Science of the total environment
|December 25, 2024
概括
环保抗生素,如四环素,可以增加Aeromonas水性对polymyxin B的耐受性,这是一个重要的抗生素. 这种由基因表达变化驱动的增加耐受性,在抗生素存在的情况下持续存在,影响水生细菌的耐药性.
科学领域:
- 微生物学 微生物学
- 环境科学 环境科学
- 水生病原体研究研究
背景情况:
- 聚米辛B是一种针对多抗药性格拉姆阴性细菌感染的关键抗生素.
- 一个重要的鱼类病原体Aeromonas hydrophila正在对polymyxin B.表现出越来越大的耐药性.
- 环境化学污染物对细菌抗生素敏感性的影响需要调查.
研究的目的:
- 为了研究环境化学污染物对水生细菌中多素B敏感性的影响.
- 为了确定短期接触特定抗生素是否会影响Aeromonas hydrophila对polymyxin B.的耐受性.
- 阐明了Aeromonas hydrophila中多素B耐受性的基础机制.
主要方法:
- 将Aeromonas hydrophila暴露在与聚米辛B同时使用的四环素,tigecycline或gentamicin的环境相关度中.
- 在抑制性多素B度下对细菌生长的评估.
- 对基因表达变化,基因型和细胞表面电荷变化的分析.
- 识别受影响的KEGG通路和失调的基因.
主要成果:
- 短时间接触到四环素,tigecycline,或 gentamicin 增加了 Aeromonas 水的耐受性对polymyxin B.
- 这种诱导的耐受性只要抗生素诱导物存在,就会持续下去.
- 增加的耐受性与改变的基因表达有关,影响诸如核糖体,新陈代谢和细胞运动等途径,没有基因型变化.
- 提高Mg2+运输和脂代谢基因的调节,降低 ompK, yidD 和 ompA 基因的调节,可能有助于增强多素 B 耐受性.
结论:
- 环境抗生素可以促进Aeromonas hydrophila中的多素B耐受性.
- 这项研究揭示了水生细菌中多素B耐受性演变的机制.
- 这些发现突显了环境抗生素污染在抗菌素耐药性上升中的作用.
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