基因和酶的结构和功能表征,涉及到与Physalis alkekengi L Lkekengi中的维他诺化物生物合成
Swati Gupta1, Bashir Akhlaq Akhoon1, Deepak Sharma1
1Genome Research Laboratory, School of Biotechnology, University of Jammu, Jammu 180006, India.
Steroids
|December 26, 2024
概括
菲萨利斯 (Physalis alkekengi L.) 提供了一种有前途的替代品,用于与诺化物生产. 维他诺化物生物合成的关键基因被保存,在叶子和花朵中表达和产生最高,这表明可持续的工业应用.
科学领域:
- 植物生物化学 植物生物化学
- 药用植物研究 药用植物研究
- 分子生物学分子生物学
背景情况:
- 菲萨利斯 (Physalis alkekengi L.) 是治疗性化合物的来源,包括维他诺化物.
- 维他诺化物是C28类类固醇乳,在传统医学中具有历史.
- 在P. alkekengi中缺乏维他诺化物生物合成基因的表征.
研究的目的:
- 在P. alkekengi. 中的withanolide生物合成途径中的关键下游基因的特征.
- 为了研究不同组织的基因表达模式.
- 为工业应用,将基因表达与植物化学概况相关联.
主要方法:
- 基因序列的表征和比较分析.
- 对保存域,二次结构和3D拓的生物信息分析.
- 在七种植物组织中进行差异基因表达分析.
- 使用高性能液体染色学 (HPLC) 的植物化学分析.
主要成果:
- 在P. alkekengi中首次对六个关键基因 (PaFPPS,PaSQS,PaSQE,PaCAS,PaHYD1,PaDWF5-1) 的表征.
- 与Withhania somnifera相比,高度保存的氨基酸序列和基本的功能领域.
- 在P. alkekengi叶子中观察到更高的基因表达水平.
- 证实了维他诺化物A和维他的生产,叶子和花朵的最大含量.
结论:
- P. alkekengi 拥有维他诺化物生物合成的保存基因,类似于 W. somnifera.
- 不同基因表达和植物化学分析表明,叶子和花朵是主要生产地点.
- P. alkekengi 是一个可行的,可持续的替代品 W. somnifera 工业 withanolide 生产,特别是 withanolide A.
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