通过优化喷雾干燥来增强Lactobacillus plantarumBG24的活力:对工艺参数,载体,冷干燥的比较分析以及存储条件的影响的洞察
Figen Kaymak Ertekin1, Özgün Köprüalan Aydın1, Özgül Altay1
1Department of Food Engineering, Faculty of Engineering Ege University İzmir Turkey.
Food science & nutrition
|December 26, 2024
概括
乳杆菌植物菌BG24益生菌的喷雾干燥 (SD) 需要最佳参数 (150°C的入口,83°C的出口) 才能实现高活力. 与SD相比,冷干燥 (FD) 提供了更高的生存率,储存温度显著影响益生菌粉末的稳定性.
科学领域:
- 食品科学 食品科学 食品科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 益生菌细菌,如Lactobacillus plantarum BG24,需要有效的保存方法来保持在食品加工期间的生存能力.
- 喷雾干燥 (SD) 是生产干燥益生菌粉的常见技术,但工艺参数和载体材料显著影响细菌的生存.
研究的目的:
- 在各种条件下,在喷雾干燥 (SD) 过程中调查 Lactobacillus plantarum BG24 的生存动态.
- 确定最佳的SD参数和载体,以最大限度地提高益生菌的生存能力.
- 为了比较SD与冷干燥 (FD) 的疗效,并评估储存条件对益生菌粉末稳定性的影响.
主要方法:
- 在不同的载体矩阵中喷雾干燥Lactobacillus plantarum BG24 (复制脱脂牛奶 (RSM),酵母提取物 (YE),大豆蛋白分离物 (SPI),阿拉伯 (GA),马尔托德克斯 (MD),砂糖 (SUC),乳糖).
- 优化SD输入和输出空气温度.
- 与SD和冷干燥 (FD) 之间的生存率的比较.
- 评估储存温度 (4°C和20°C) 对粉末特性 (含水量 (MC),水活性 (a_w)) 和细胞活性的影响.
主要成果:
- 最佳的SD参数 (150°C入口,83°C出口) 产生了高可行性 (92.23%),低MC (3.57%) 和低a_w (0.266).
- 单独的RSM,RSM + GA和单独的SPI被确定为SD期间益生菌生存的优越干燥载体.
- 在使用RSM + YE作为载体时,冷干燥 (FD) 显示了较高的存活率 (97.69%) 与SD相比.
- 储存温度的提高对细胞活力产生了负面影响,特别是对于SD益生菌粉末.
结论:
- 优化喷雾干燥条件和适当的载体选择对于保持益生菌生存能力至关重要.
- 与喷雾干燥相比,冷干燥为实现高益生菌生存率提供了更有效的方法.
- 储存温度在保持干燥益生菌粉末的稳定性和活力方面发挥着关键作用,较低的温度更有利.
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