在乳制品中的细菌分离物的体外查,以检测益生菌特性和其他促进健康的属性
Ishita Modasiya1, Priya Mori1, Hina Maniya1
1Postbiotics and Foodomics Lab, Department of Microbiology, School of Science RK University Rajkot Gujarat India.
Food science & nutrition
|December 26, 2024
概括
在古吉拉特州,从当地乳制品中分离出六种有前途的益生菌菌株. 这些细菌在酸性和胆汁条件下表现出极好的生存能力,强大的自聚和高细胞表面水性,以及显著的抗氧化活性.
科学领域:
- 微生物学 微生物学
- 食品科学 食品科学 食品科学
- 生物技术是生物技术.
背景情况:
- 益生菌微生物提供健康益处,但需要严格的查有效性.
- 乳制品是潜在益生菌候选人的丰富来源.
- 当地采购确保了对区域粮食系统的相关性.
研究的目的:
- 从本土乳制品中分离和描述新型益生菌菌株.
- 为了评估所选单元的生存,粘附和抗氧化能力.
- 用分子方法识别有前途的菌株,以确定潜在的应用.
主要方法:
- 从Saurashtra乳制品中选224个殖民地,以检测益生菌属性.
- 70个分离物的二次选,随后对23个分离物的抗氧化活性测试.
- 16S rRNA测序用于物种识别和抗氧化剂测定 (ABTS,DPPH,FRAP).
主要成果:
- 六个分离物满足了所有初级和二级益生菌标准,对低pH和胆汁有很高的耐受性.
- 分离体显示出显著的自身聚合 (≥81.08%) 和细胞表面水性 (>70%).
- 鉴定的菌株属于Bacillus,Enterococcus和Lactobacillus属,具有显著的抗氧化能力.
结论:
- 该研究成功地发现了来自当地乳制品来源的六种强大的益生菌菌株.
- 这些菌株具有理想的益生菌特性和抗氧化特性.
- 已识别的Bacillus,Enterococcus和Lactobacillus分离物是功能性食品开发的有希望的候选者.
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