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Updated: Aug 9, 2026

Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction
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Preparation of Formalin-fixed Paraffin-embedded Tissue Cores for both RNA and DNA Extraction

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优化RNA提取方法,用于高通量转录组测序的甲固定嵌心脏组织样本

Nkechi Martina Odogwu1, Jin Sung Jang2,3, Sabrina Albertson1

  • 1Program for Hypoplastic Left Heart Syndrome, Mayo Clinic Rochester, Rochester, Minnesota, United States of America.

PloS one
|December 26, 2024
PubMed
概括

从FFPE心脏组织中优化RNA提取是生物标志物发现的关键. 方法QE和BL显示出优异的RNA产量和质量,使得心血管疾病研究的高分辨率转录组分析成为可能.

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相关概念视频

RNA-seq03:21

RNA-seq

RNA sequencing, or RNA-Seq, is a high-throughput sequencing technology used to study the transcriptome of a cell. Transcriptomics helps to interpret the functional elements of a genome and identify the molecular constituents of an organism. Additionally, it also helps in understanding the development of an organism and the occurrence of diseases. 
Before the discovery of RNA-seq, microarray-based methods and Sanger sequencing were used for transcriptome analysis. However, while microarray-based...