基因型测序揭示了一种Thinopyrum 4StS·1JvsS 罗伯逊式转位,与面包小麦的多重应力耐受性有关
Klaudia Kruppa1, Edina Türkösi2, Kateřina Holušová3
1Hungarian Research Network (HUN-REN), Centre for Agricultural Research, Agricultural Institute, Martonvásár, 2462, Hungary.
概括
一个新的小麦系 (GLA7) 具有独特的罗伯逊转位,可以抵抗生病并增强干旱耐受性. 这种遗传资源保持了产量稳定性,为改善小麦品种提供了巨大的潜力.
科学领域:
- 植物遗传学和育种.
- 提高作物质量 提高作物质量
- 基因组学就是基因组学.
背景情况:
- 对Agropyron glael (GLAEL) 染色体结构的有限理解阻碍了其在小麦育种中的应用.
- 格莱尔,一个Thinopyrum中间体 × Th. 的混合物,是小麦改进的宝贵遗传资源.
- 关于GLAEL和六倍体小麦 (Triticum aestivum) 之间的同质关系存在知识差距.
研究的目的:
- 开发和描述一个小麦/GLAEL 44 染色体添加线 (GLA7).
- 调查GLAEL对小麦的染色体组成和遗传贡献.
- 评估GLA7在增强小麦对疾病和非生物压力的抵抗力方面的潜力.
主要方法:
- 一个小麦/GLAEL 44 染色体添加线 (GLA7) 的发展.
- 多色基因组在位杂交 (GISH) 用于染色体鉴定.
- 基因型测序 (GBS) 阅读覆盖范围分析用于转位识别.
- 对抗疾病 (叶子和条纹生) 和耐旱性进行表型化.
- 实地试验以评估农学性能和产量.
主要成果:
- 使用GBS在GLA7中发现了一种独特的罗伯逊转位 (4StS·1JvsS),涉及两个Thinopyrum亚基因组,使用GBS在GLA7中进行了鉴定.
- GLA7表现出强大的成年植物对叶子生和条纹生的抵抗力.
- GLA7表现出增强的干旱耐受性,保持了发芽形态和水含量.
- 实地试验证实,GLA7的农学性能和产量稳定性与家长小麦系相美.
结论:
- 开发的小麦/GLAEL添加线 (GLA7) 携带一种新的罗伯逊式转移,赋予有价值的特征.
- 在小麦中,GLA7提供了对生疾病的增强抵抗力和改善的干旱耐受性.
- 这种加种线代表了一种有前途的遗传资源,可以在不影响产量的情况下开发适应气候变化的小麦品种.
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