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肠道小杯细胞表达的Reg4通过抑制致病性大肠杆菌感染来改善潜在的肠道炎症

Ying Lu^1,2,3, Bo Wu^1,2, Weipeng Wang^4,2

¹Division of Pediatric Gastroenterology and Nutrition, Xinhua Hospital, School of Medicine, Shanghai Jiao Tong University, No. 1665, Kong Jiang Road, Shanghai, China.

Probiotics and antimicrobial proteins

|December 26, 2024

概括

再生小岛衍生家族成员4 (Reg4) 通过抑制致病性大肠杆菌生长来保护肠道炎症. 这一发现为炎症性肠病 (IBD) 机制提供了新的见解.

科学领域:

胃肠病学胃肠病学
微生物学微生物学
免疫学免疫学免疫学

背景情况:

增加大肠杆菌 (大肠杆菌) 的数量与炎症性肠病 (IBD) 的进展相关.
在肠道炎症中再生小岛衍生家族成员4 (Reg4) 的作用仍然不清楚,尽管它与性结肠炎 (UC) 患者隔离.

研究的目的:

通过使用条件淘汰赛小鼠模型,研究Reg4在肠道炎症中的功能和机制.
阐明Reg4在对肠病原性大肠杆菌 (EPEC) 感染和硫酸 (DSS) 诱导的大肠炎的反应中的作用.

主要方法:

生成的肠道条件Reg4淘汰赛小鼠 (Reg4^ΔIEC).
使用了EPEC感染肠道和DSS诱导大肠炎的小鼠模型.
评估了肠道微生物群的变化,Reg4蛋白的给药效果,以及体外EPEC抑制.

主要成果:

在儿科IBD肠道粘膜中,Reg4被上调,特别是在杯状细胞中.
在小鼠中,Reg4缺乏增加了蛋白质细菌和肠杆菌群的丰富性.
重组Reg4蛋白减少了EPEC诱导的炎症和损伤;它抑制了EPEC的生长,运动,粘附和入侵.
雷格4的曼南结合点对于其抗菌活性至关重要.
雷格4淘汰赛小鼠对DSS诱导的大肠炎的易感性增加,这种易感性通过 gentamicin 治疗得到改善.

结论:

来自肠道杯细胞的Reg4对于预防实验性结肠炎至关重要.
Reg4对EPEC具有杀菌活性,突出显示其在肠道炎症中的保护作用.

关键词:

埃舍里希亚大肠杆菌 (Escherichia coli) 是一个大肠杆菌.肠道杯细胞肠道杯细胞肠道炎症是一种肠道炎症.在 Reg4 中,您可以在 Reg4 中使用 Reg4 文件.

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