一个红光可激活的内源基因转录系统与红CPTSTS
Takahiro Nakajima1,2, Yuto Kuwasaki2, Shota Yamamoto2
1Kanagawa Institute of Industrial Science and Technology, Kawasaki-shi, Kanagawa, Japan.
Methods in molecular biology (Clifton, N.J.)
|December 26, 2024
概括
我们开发了MagRed,一个可通过红光激活的光开关,使深层组织光遗传学成为可能. 这个名为Red-CPTS的系统使用CRISPR-Cas9来精确控制细胞和活小鼠的基因转录.
科学领域:
- 视觉遗传学 视觉遗传学
- 分子生物学分子生物学
- 基因编辑 基因编辑
背景情况:
- 红光提供深层组织透和低光毒性,非常适合生物应用.
- 现有的光遗传工具在深层组织中往往缺乏效率.
- 克里斯普尔-Cas9技术提供了精确的基因组操纵能力.
研究的目的:
- 开发一种新的红光可激活光开关 (MagRed),用于增强深层组织光遗传学.
- 创建一个基于CRISPR-Cas9的系统 (Red-CPTS) 用于红光诱导的内源基因转录.
- 为在哺乳动物细胞和体内实行红色CPTS提供详细的协议.
主要方法:
- 发展MagRed光开关的发展.
- 集成MagRed与CRISPR-Cas9形成红色CPTS系统.
- 在培养的哺乳动物细胞和活小鼠中建立基因激活协议.
主要成果:
- 成功开发了MagRed,一个可通过红光激活的光开关.
- 创建Red-CPTS系统用于红光控制的基因转录.
- 在细胞培养和体内小鼠模型中证明Red-CPTS的疗效.
结论:
- 马格红和红CPTS为深层组织光遗传学提供了一个强大的新工具.
- 这种系统能够精确控制内源基因转录的红光诱导性控制.
- 提供的协议有助于在各种研究环境中应用Red-CPTS.
相关概念视频
Reporter Genes
11.0K
Reporter genes are a type of protein-coding gene that are often tagged to a gene of interest. Once inside a target cell, reporter genes usually produce visually identifiable characteristics like fluorescence and luminescence when expressed along with the gene of interest. Thus, reporter genes “report” the presence or absence of genes of interest in an organism, determine the gene expression pattern, or track the physical location of a DNA segment or protein in the cell.
11.0K
Conservative Site-specific Recombination and Phase Variation
5.9K
Because the DNA segments are cut and reorganized in a direction-specific manner, site-specific recombination has emerged as an efficient genetic engineering technique. Flippase and Cyclization recombinases or Flp and Cre, respectively, are two members of the tyrosine recombinase family derived from bacteriophages, that are used to mediate site-specific DNA insertions, deletions, and targeted expression of proteins in mammalian cell lines.
The recognition sites for Cre recombinase called LoxP...
The recognition sites for Cre recombinase called LoxP...
5.9K
Channel Rhodopsins
2.5K
Most organisms use photoreceptors to sense and respond to light. Examples of photoreceptors include bacteriorhodopsins and bacteriophytochromes in some bacteria, phytochromes in plants, and rhodopsins in the photoreceptor cells of the vertebral retina. The light-sensitive property of these receptors is because of the bound chromophores, such as bilin in the phytochromes and retinal in the rhodopsins.
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
Rhodopsins belong to the family of cell surface proteins called G-protein coupled receptors,...
2.5K


