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使用联合免疫组织化学和免疫光技术解决了两起致命的胰岛素杀伤病例

Yiling Li¹, Zhonghao Yu¹, Yuhao Yuan¹

¹Department of Forensic Medicine, Tongji Medical College, Huazhong University of Science and Technology, No. 13 Hangkong Road, Wuhan, 430030, People's Republic of China.

Forensic science, medicine, and pathology

|December 26, 2024

概括

致命的胰岛素过量服用很难在死后检测到. 这项研究在注射部位和体液中确定了胰岛素和原胺,有助于在这些具有挑战性的病例中进行法医识别.

科学领域:

法医毒理学法医毒理学
内分泌学在内分泌学.
病理学病理学病理学

背景情况:

糖尿病管理通常涉及合成胰岛素治疗.
过度注射胰岛素可能导致致命的结果.
致命胰岛素过量剂量的法医鉴定是具有挑战性的,因为快速的药理动力学和微妙的病理变化.

研究的目的:

研究法医识别致死性胰岛素过量剂量的方法.
在疑似过量胰岛素的情况下,分析尸体样本.

主要方法:

从两起致命的胰岛素过量服用病例中收集并分析了死后标本.
使用免疫组织化学和免疫光测试来检测胰岛素和原胺.
在各种生物样本上进行毒理学分析.

主要成果:

胰岛素和原胺在注射部位始终被发现,分布在皮肤层和相关细胞中.
在表皮,皮下脂肪,血液,尿液,玻璃体和胆汁中检测到胰岛素颗粒.
检测是成功的,即使死后的间隔长达28天.

结论:

在注射部位和全身样本中存在胰岛素和原胺,为法医鉴定提供了关键证据.
这项研究提供了有价值的见解和指导方针,用于在法医调查中诊断致命的胰岛素过量.

关键词:

免疫光效应免疫光效应免疫组织化学免疫组织化学过量的胰岛素过量服用.蛋白质胺是一种原蛋白.

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