转基因-2-hexenal通过氧化损伤Neofusicoccum parvum细胞膜来减少收获后的果茎末部腐烂
Xiaoli Tan1, Xiaobing Jiang1, Xiumei Chen1
1School of Chemical Engineering, Xiangtan University, Xiangtan, 411105, China.
World journal of microbiology & biotechnology
|December 26, 2024
概括
植物精油成分Trans-2-hexenal (TH) 有效地控制了果腐烂病原体Neofusicoccum parvum. 它通过诱导活性氧物种 (ROS) 破坏细胞膜,为果提供了有前途的天然防腐剂.
科学领域:
- 植物病理学 植物病理学
- 菌类学 菌类学是指菌类学.
- 食品科学 食品科学 食品科学
背景情况:
- 新虫 (Neofusicoccum parvum) 是收获后果果腐烂的主要原因之一.
- 需要有效的控制策略来最大限度地减少经济损失.
研究的目的:
- 评估转化-2-hexenal (TH) 作为一种潜在的抗真菌剂对抗N. parvum.
- 阐明TH的作用机制.
- 开发一个改进的TH的交付系统.
主要方法:
- 在体外和体外的抗真菌测定对N. parvum.
- 细胞膜完整性的分析 (化的吸收,脂质和厄戈斯特醇含量).
- 使用DCFH-DA染色测量反应性氧物种 (ROS) 生产.
- 开发和测试气凝装载TH (ALTH) 材料,用于果实应用.
主要成果:
- 在0.4μL/mL的度下,TH在体外对N. parvum表现出强大的抗真菌活性.
- TH破坏了细胞壁和膜的完整性,增加了酸的吸收,减少了脂质/厄戈斯特醇.
- 在N. parvum mycelia中,TH诱导了ROS积累和脂质过氧化.
- ALTH在体外显示出出色的抗真菌活性,并在体内显著减轻果茎末端腐烂.
- 40μL/L的ALTH在有效性方面与普洛克罗拉兹相当,可以保持水果的质量.
结论:
- 通过ROS积累诱导细胞膜的氧化损伤,Trans-2-hexenal有效地控制N. parvum.
- 装有气凝的TH (ALTH) 是一个有前途的配方,可用于实际应用.
- TH代表了一种可行的天然防腐剂,可以防止收获后的果腐烂.
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