在微藻-细菌联盟的二次废水中,甲和硫甲的生物降解
Daniel Aparecido da Silva Rodrigues1, Camila Cristina Rodrigues Ferreira da Cunha1, Andressa Rezende Pereira2
1Multicenter Postgraduation Program in Chemistry, Minas Gerais, Federal University of Ouro Preto (UFOP), Ouro Preto, 35450-000, Minas Gerais, Brazil.
International journal of hygiene and environmental health
|December 26, 2024
概括
一个微藻-细菌联盟有效地从废水中去除了三甲 (TMP) 和硫 (SMX),尽管其速率低于预期. 这种共生生物降解方法对解决废水中抗生素耐药性的有希望.
科学领域:
- 环境微生物学 环境微生物学
- 水处理技术水处理技术
- 抗微生物耐药性 抗微生物耐药性
背景情况:
- 三甲 (TMP) 和硫 (SMX) 是经常在废水污水中检测到的抗生素.
- 传统的废水处理厂 (WWTP) 在去除这些制药污染物方面是无效的.
- 抗生素排放有助于抗菌素耐药性的扩散,构成公共卫生风险.
研究的目的:
- 评估微藻-细菌联盟作为四级处理方法的有效性,用于从真实WWTP废水中去除TMP和SMX.
- 评估硫胺抗性基因 (sul1) 与抗生素降解同时被去除的情况.
- 为了研究没有营养丰富的去除潜力,专注于共生生物降解.
主要方法:
- 在低强度LED照射下培养天然微藻-细菌联盟.
- 该联盟的应用作为对真正的WWTP二次废水的第三级处理,其中添加了TMP/SMX (每种50μg L-1).
- 使用低温分离提取 (LTPE) 和HPLC-ESI-MS/MS.的剩余抗生素的量化.
- 通过定量聚合酶链反应 (qPCR) 分析sul1基因丰度.
主要成果:
- 微藻-细菌联盟证明了TMP (24.58%) 和SMX (48.34%) 的去除,而SMX去除更有效.
- 抗生素度没有抑制微藻-细菌联盟的生长.
- 在7天后,观察到sul1和16SrRNA基因丰度略有增加.
- 共生生物降解被确定为主要的去除机制,生物吸附,生物积累或非生物因素的贡献最小.
结论:
- 微藻-细菌联盟显示出作为一种可持续的四级处理方法,用于从废水中去除抗生素的潜力.
- 需要进一步优化,以提高降解率,并根据监管标准实现超过80%的去除效率.
- 这种方法提供了一个有希望的,独立于营养素的策略,以减轻抗生素和耐药基因在环境中的传播.
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