使用人类肠道肠状细胞培养物来检测多种胃肠病毒
Michael Kulka1, Brianna Keinard1, Stanislav V Sosnovtsev2
1Office of Applied Microbiology and Technology, Office of Laboratory Operations and Applied Science, Human Foods Program, Food and Drug Administration, Laurel, MD 20708, USA.
Journal of virological methods
|December 26, 2024
概括
人类肠道体支持人类诺罗病毒和天体病毒的共同复制. 干扰宿主抗病毒反应可以增强共感染期间的诺病毒复制,帮助食品安全检测工作.
科学领域:
- 病毒学 病毒学
- 胃肠病学 胃肠病学
- 食品安全 食品安全
背景情况:
- 人类诺病毒 (HuNoV) 和人类天体病毒 (HAstV) 是导致急性胃肠炎的关键原因.
- 在食品和环境样本中检测传染性肠道病毒对于公共卫生至关重要.
- 目前的培养方法对于许多肠道病毒是有限的,这阻碍了感染性评估.
研究的目的:
- 评估人类肠道肠 (HIEs) 作为HuNoV和HAstV.的共同复制模型.
- 研究共感染对病毒复制和宿主免疫反应的影响.
主要方法:
- 用单个HuNoV GII.4[P16]和HAstV3.3株感染HIEs的感染.
- 与不同病毒度的同时感染的实验.
- 病毒RNA水平的量化和病毒囊蛋白的检测.
- 对宿主干扰素 (IFN) 反应的测量和对抗IFN抗体的测试.
主要成果:
- 高位病毒支持HuNoV GII.4[P16]和HAstV3.3的复制.
- HAstV3复制达到24hpi的峰值,而HuNoV GII.4[P16]达到48-72hpi的峰值.
- 同时感染HAstV3抑制了HuNoV复制,但不是反之亦然.
- 干扰素反应是由HAstV3感染引起的.
- 中和抗IFN抗体在联合感染期间部分恢复了HuNoV复制.
结论:
- 人类肠道肠杆菌是研究不同肠道病毒 (如HuNoV和HAstV) 共同复制的可行模型.
- 主体抗病毒机制,如干扰素反应,影响病毒共同复制动态.
- 准宿主抗病毒通路可能会提高复杂样本中特定病毒的检测.
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