提高了黄素的可溶性,稳定性,生物可访问性和抗氧化活性,使用化豆蛋白基纳米细胞:pH驱动方法与乙醇诱导方法
Jiang Yi1, Ling Kang1, Dixue Luo1
1Shenzhen Key Laboratory of Food Macromolecules Science and Processing, College of Chemistry and Environmental Engineering, Shenzhen University, Shenzhen 518060, China.
International journal of biological macromolecules
|December 26, 2024
概括
豆蛋白纳米粒有效封装黄素,增强其溶解性,稳定性和抗氧化特性. 以pH驱动的方法 (PDM) 证明优于以乙醇诱导的方法 (EIM) 来创建这些有价值的营养药物输送系统.
科学领域:
- 食品科学与技术 食品科学与技术
- 材料科学 材料科学 材料科学
- 生物技术是生物技术.
背景情况:
- 黄素 (CUR) 是一种疏水化合物,具有较差的水溶性和稳定性,限制了其在营养保健品中的使用.
- 豆蛋白水解剂 (PPHs) 由于其生物相容性和功能,具有作为天然纳米载体系统的潜力.
- 开发有效的封装策略对于改善黄素等生物活性化合物的输送和生物可用性至关重要.
研究的目的:
- 开发和比较两种方法 (PDM和EIM) 在豆蛋白纳米粒中封装黄素.
- 为了评估含库尔库明的纳米微粒的物理化学特性,负载能力,稳定性和生物可访问性.
- 评估封装黄素的体外抗氧化活性,并比较两种制造方法的疗效.
主要方法:
- 使用Protamex酶生成水解剂的豆蛋白的部分水解.
- 使用pH驱动方法 (PDM) 和乙醇诱导方法 (EIM) 制造纳米粒.
- 纳米微粒 (形状,大小分布) 和黄素加载效率的表征.
- 使用光学光谱学评估黄素-PPH相互作用.
- 评估水溶性,储存稳定性 (紫外线,热),生物可访问性和抗氧化活性.
主要成果:
- 无论是PDM还是EIM都产生了具有均颗粒大小的球形纳米粒.
- 与EIM相比,pH驱动方法 (PDM) 实现了较高的黄素加载量 (3.21%) .
- 黄素封装显著提高了水溶性,紫外线/热稳定性,生物可访问性和抗氧化活性.
- 通过PDM制造的纳米微粒表现出优越的光稳定性,生物可访问性和抗氧化活性,而不是EIM制造的纳米微粒.
结论:
- 豆蛋白纳米粒是稳定和增强黄素特性的有效载体.
- 以pH驱动的方法 (PDM) 是一种比以乙醇诱导的方法 (EIM) 更有效的方法,用于制造高性能素载入的纳米微粒.
- 这些发现为在营养药应用中利用基于植物蛋白的输送系统提供了宝贵的见解.
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