在供水固态发酵中,Trichoderma reesei改善细胞酶生产的机制
Wenjing Xue1, Yiru Sheng1, Yukai Tai1
1Sanya Institute of Nanjing Agricultural University, Department of Microbiology, Key Lab of Microbiological Engineering of Agricultural Environment, Ministry of Agriculture, College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, PR China.
Bioresource technology
|December 26, 2024
概括
超吸收性聚合物 (SAP) 在供水固态发酵 (SSF) 中增强细胞酶的产生. 这种方法可以增强酶活性和基因表达,克服了纤维素酸应用中的局限性.
科学领域:
- 生物技术是生物技术.
- 生物化学工程 生物化学工程
背景情况:
- 纤维素的高生产成本阻碍了林氏纤维素的商业化.
- 固态发酵 (SSF) 提供了水和能源的节约,但面临着水的可用性和损失的挑战.
研究的目的:
- 研究使用超吸收聚合物 (SAP) 来增强细胞酶生产的构建供水SSF的有效性.
- 阐明SAPs改善细胞酶活性和基因表达的潜在机制.
主要方法:
- 采用了与Trichoderma reesei Rut-C30一起建造的供水SSF系统.
- 在发酵过程中监测基质物理性能和水特性.
- 进行了转录组分析,以评估基因表达变化.
主要成果:
- 实现了碳氧甲基纤维素活性增加1.7倍,过纸活性增加2.3倍.
- 证明SAP介导的供水改善了基质利用和早期细胞活动.
- 观察到细胞酶活动相关基因的显著上调,包括甘氨酸酸酶和主要促进者超级家族.
结论:
- 通过SAP的供水有效地提高了SSF中的细胞质生产.
- SAPs通过提高基质利用率,增加细胞活性和调节关键酶相关基因来促进细胞酶的产生.
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