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编码MYB-CC转录因子的GsMYB10增强了大豆对酸性应激的耐受性

Ce Yang^1,2, Xiang Lu^1,3, Dan Du^2,3

¹The State Key Laboratory for Conservation and Utilization of Subtropical Agro-bioresources, South China Agricultural University, Guangzhou, Guangdong, 510642, China.

BMC plant biology

|December 26, 2024

概括

这项研究确定GSMYB10是提高耐受性的关键大豆基因. 过度表达GsMYB10可以改善根生长,并减少在酸性压力下的积累.

科学领域:

植物分子生物学植物分子生物学
植物生理学植物生理学
遗传学遗传学是一个

背景情况:

MYB转录因子 (TFs) 对植物应激反应至关重要.
MYB-CC子家族,包括来自大豆的GsMYB10,对耐受性的研究是有限的.

研究的目的:

从野生大豆中克隆和特征GsMYB10基因.
调查GSMYB10在大豆中赋予耐受性的作用.

主要方法:

从野生大豆BW69中克隆GsMYB10基因.
在酸性应力下进行表达分析.
对GsMYB10蛋白质的核定位和转录活性测定.
在应力下对GsMYB10过度表达 (OE) 的转基因大豆植物进行表型分析.
测量含量,素和甲.
转录组分析以确定差异表达基因 (DEGs).

主要成果:

GsMYB10表达是由酸性应力诱导的,并且是大豆根的组成部分.
GsMYB10蛋白质具有MYB和卷轴-卷轴 (CC) 域,局部化到核,并表现出转录活性.
在压力下,GsMYB10-OE植物表现出增强的尖根长度和根新鲜重量.
与野生类型相比,转基因植物在根尖积累的 (Al3+) 明显较少.
在GsMYB10-OE植物中,proline含量增加,malondialdehyde含量减少.
过度表达GsMYB10调节了与细胞壁修饰相关的DEGs,NRAMPs和ABC载体的表达.

结论:

GsMYB10 作为大豆中耐受性的积极调节者.
这种基因增强了大豆适应酸性压力的能力.
这些发现有助于了解大豆对毒性的反应机制.

关键词:

的应力压力是什么在MYB-CC家族中,MYB-CC是最重要的.豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆豆转录因子是一种转录因子.

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