在绿豆中RNA干扰分子的有效性和命运,Acyrthosiphon pisum
Arinder K Arora1, David S Kang2
1Department of Entomology, Texas A&M University, College Station, Texas, USA.
Archives of insect biochemistry and physiology
|December 27, 2024
概括
转基因植物可以提供RNA干扰 (RNAi) 生物杀虫剂来对抗虫害. 双链RNAs (dsRNAs) 和小干扰RNAs (siRNAs) 都有效地降低了害虫基因表达,污染风险低.
科学领域:
- 农业科学 农业科学
- 分子生物学分子生物学
- 昆虫学 昆虫学是一门学科.
背景情况:
- RNA干扰 (RNAi) 为农业中的昆虫害虫控制提供了一种新的方法.
- RNAi利用双链RNAs (dsRNAs) 沉默特定的昆虫基因,减少害虫种群.
- 目前的RNAi应用包括叶子喷雾和转基因植物表达.
研究的目的:
- 调查使用转基因植物提供基于RNAi的生物杀虫剂对虫害物的可行性.
- 评估dsRNAs和siRNAs在减少虫的基因表达中的有效性.
- 评估与RNAi生物农药持久性相关的潜在环境风险.
主要方法:
- 转型的Arabidopsis thaliana植物被设计成在Cucumis melo galactinol合成酶1促进剂下表达dRNA.
- 虫 (Acyrthosiphon pisum) 通过口服暴露于dSRNA或siRNA.
- 用转录分析量化了虫中aquaporin 1基因的基因表达水平.
- 在暴露后监测昆虫和蜂蜜中的dsRNAs和siRNAs的持久性.
主要成果:
- 转型的阿拉比多普西斯伴侣细胞在可检测的水平上表达dRNA,但不是siRNA.
- 口服dSRNAs和siRNAs导致 pea aphids 中的水素1转录的可比降低.
- 暴露后48小时内,在昆虫或蜂蜜中没有检测到dsRNA或siRNA.
结论:
- 转基因植物显示出作为RNAi生物杀虫剂对虫害的传递系统的承诺.
- dsRNAs和siRNAs都有效地在口服时使虫的基因沉默.
- RNAi分子的快速降解表明,除了目标害虫之外,环境污染的风险很低.
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