储存和提取方法对泥炭土壤微生物群的影响
Dylan Cronin1,2, Yueh-Fen Li2, Paul Evans3
1Center of Microbiome Science, Ohio State University, Columbus, Ohio, United States.
PeerJ
|December 27, 2024
概括
样品储存和核酸提取方法显著影响泥炭土微生物群落结构. 方法的一致性对于可靠的微生物组研究和气候反解释至关重要.
科学领域:
- 环境微生物学 环境微生物学
- 土壤科学 土壤科学
- 分子生态学分子生态学
背景情况:
- 泥炭土是关键的碳储库,其中的微生物组影响气候反.
- 微生物社区结构恢复对样品处理和加工非常敏感.
- 了解方法影响对于准确的泥炭地微生物组研究至关重要.
研究的目的:
- 评估不同储存条件和核酸提取方法对泥炭土微生物群落的影响.
- 从不同的泥炭地息地中识别微生物群恢复的基于方法的偏差.
主要方法:
- 测试了三个现场储存条件 (LifeGuard,快速结,冰上运输),然后进行-80°C的储存.
- 将五种不同的核酸提取协议应用于来自三个Stordalen Mire息地的泥炭土样本.
- 分析了细菌和考古社区结构,使用安普利康测序.
主要成果:
- 最初的储存方法影响了α多样性,但没有影响β多样性.
- 核酸提取方法显著影响了阿尔法和β多样性.
- 一个提取套件 (PowerSoil总RNA隔离套件与DNA脱离配件套件) 与其他套件相比,产生了不同的微生物种类和社区结构.
结论:
- 方法选择引入了泥炭土微生物群恢复的重大偏差.
- 息地和样本深度仍然是主要因素,但方法效应显著.
- 在泥炭地研究中,标准化的方法对于强大的研究间比较和生态解释至关重要.
相关概念视频
Methods to Assess Microbial Communities
60
Microbial communities, comprising bacteria, archaea, and eukaryotic microorganisms, inhabit diverse ecosystems and play crucial roles in environmental and biological processes. Their diversity is defined by three main parameters: species richness (the number of distinct species), species abundance (the relative quantity of each species), and species evenness (how uniformly individual species are distributed in various locations). These factors together shape the structure and ecological balance...
60
Microenvironments
54
Microorganisms inhabit highly localized spaces known as microenvironments, which are defined by distinct physical and chemical characteristics. These include oxygen concentration, pH, temperature, light availability, and nutrient levels. The conditions within a microenvironment can differ markedly from those in the surrounding area and significantly influence microbial growth, metabolism, and community structure.Microenvironments often display sharp physicochemical gradients over small spatial...
54
Soil Microbial Ecology
83
Soil microbial ecology is defined by highly diverse, spatially structured communities that drive nutrient cycling, organic matter turnover, and overall ecosystem stability. Although a gram of soil can contain thousands of bacterial and archaeal taxa, the ecological processes they mediate are even more crucial for sustaining terrestrial life.Microhabitats and NichesSoil is a heterogeneous mixture of minerals, organic matter, water, and air. Microbes inhabit distinct microhabitats formed by...
83
Microbes and Climate Change
91
Microorganisms are pivotal agents in Earth's biogeochemical cycles, significantly influencing climate dynamics through their metabolic activities. These microbes modulate the levels of key greenhouse gases by both contributing to and helping mitigate climate change.Microbial Contributions to Greenhouse Gas EmissionsRising global temperatures accelerate microbial metabolism, which, in turn, speeds up the decomposition of organic matter. This process releases carbon dioxide (CO₂) through...
91


