在Saccharomyces cerevisiae和Bacillus subtilis之间进行受控的跨王国细胞-细胞通信,使用定数感应
Tomislav Vološen1, Uta Gutbier2,3, Ramón Korn2
1General Microbiology, Chair of General Microbiology, TUD Dresden University of Technology, Dresden, Germany.
Frontiers in microbiology
|December 27, 2024
概括
研究人员设计了一种Bacillus subtilis记者菌株,以检测酵母菌的通信信号. 这为生物技术应用建立了一个新的跨王国信号系统.
科学领域:
- 合成生物学 合成生物学
- 微生物的沟通方式
- 生物技术是生物技术.
背景情况:
- 控制的物种间通信对于未来的生物技术和生物反应器应用至关重要.
- 定数感应介于微生物的通信,如细菌细菌.
- 在 prokaryotes 和 eukaryotes 之间建立跨王国信号仍然是一个挑战.
研究的目的:
- 设计一种Bacillus subtilis报告员系统,用于检测来自酵母的特定的定数感应.
- 在细菌和酵母之间建立一个受控的王国间通信系统.
- 提高这种通信系统对潜在应用的敏感性和稳定性.
主要方法:
- 工程 Bacillus subtilis 记者菌株使用Coma-依赖的srfAA促进体融合到记者基因.
- 利用了来自B. subtilis.的酸酶RapF (PhrF) 的能力和化因子 (CSF) 和活性调节器.
- 在Saccharomyces cerevisiae中异质地产生,并使用微板阅读器监测记者活动.
- 进行了系统性突变发生,以增强促进体活性和敏感性.
- 通过在液体和固体介质中的共同培养研究验证了信号传输.
主要成果:
- 改造的B. subtilis记者菌株特别检测到CSF和PhrF.
- 突变酶增加了srfAA促进体活性,增强了对酵母产生的的记者敏感性.
- 同种植证明了强大的信号传输,促进器活动增加了多达5倍.
- 成功建立了群体感知驱动的酵母和细菌之间的跨王国交声.
结论:
- 酵母和细菌之间成功建立了一个新的跨王国通信系统,使用定数感应.
- 该系统为共同培养中受控蛋白质表达和生物传感器执行器系统的开发提供了基础.
- 工程记者菌株有助于研究多种生物反应器中的细胞相互作用和代谢物交换.
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