通过抑制病原体和使用微生物的宿主耐药性增强管理番茄细菌枯
Ishan Tiwari1, Ali Asger Bhojiya2, Devendra Jain3
1Amity Institute of Microbial Technology, Amity University Uttar Pradesh, Noida, India.
Frontiers in microbiology
|December 27, 2024
概括
来自Lactiplantibacillus argentoratensis的新型抗微生物 (AMP) 有效控制番茄植物中的细菌枯. 这种生物合成的可以减少疾病,促进植物生长,为化学农药提供可持续的替代品.
科学领域:
- 农业科学 农业科学
- 微生物学 微生物学
- 生物技术是生物技术.
背景情况:
- 合成农药带来健康和环境风险,推动了对可持续植物保护的需求.
- 由Ralstonia solanacearum引起的细菌 (BW) 严重影响番茄 (Solanum lycopersicum) 的生产.
- 抗微生物 (AMP) 是一种有前途的自然生物控制剂.
研究的目的:
- 为了研究生物合成的AMP从Lactiplantibacillus argentoratensisIT对西红中的Ralstonia solanacearum的疗效.
- 评估AMP的直接抗菌活性及其在诱导植物防御机制方面的作用.
- 评估AMP应用对番茄植物生长和形态学的影响.
主要方法:
- 在叶子上应用生物合成的AMP (PRKGSVAKDVLPDPVYNSKLVTRLINHLMIDGKRG) 在200 ppm.
- 评估番茄植物中细菌枯的疾病发病率和严重程度.
- 扫描电子显微镜用于观察细菌细胞形态变化.
- 测量植物防御酶活动和植物生长参数 (长度,生物量,根长度).
主要成果:
- 叶子AMP应用显著减少了49.3%的细菌发病率和45.8%的严重程度.
- 扫描电子显微镜证实了Ralstonia solanacearum细胞的严重形态损伤.
- AMP治疗增强了宿主抵抗力,增加了植物长度 (95.5%),生物量 (20.1%) 和根长度 (96.69%).
结论:
- 生物合成的AMP具有双重功能:对R. solanacearum的直接抗菌作用和诱导番茄植物防御反应.
- 这种AMP代表了一种新,有效和可持续的生物控制剂,用于控制Solanum lycopersicum中的细菌.
- 这项研究是首次报告生物合成微生物的叶子应用,用于生物控制R. solanacearum和促进番茄生长.
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