调节巨细胞和黑色素瘤癌细胞之间的外体通信,通过载有多克索鲁比的基于环氧的纳米海绵进行调节
Mohammad Mahmoudian1,2, Shokoufeh Alizadeh2, Darya Lotfi2
1Candiolo Cancer Institute, FPO-IRCCS, University of Turin, Candiolo, Italy.
Nanotoxicology
|December 27, 2024
概括
含有多克索鲁比的纳米海绵增强了癌细胞的毒性,但改变了巨细胞外体,促进癌细胞生长. 赛克洛德克斯特林纳米海绵可能会增加多克索鲁比辛的有效性,同时影响瘤微环境通信.
科学领域:
- 在瘤学瘤学.
- 纳米医学是一种纳米医学.
- 细胞生物学 细胞生物学
背景情况:
- 瘤微环境 (TME) 涉及癌症和非恶性细胞,外体细胞介导细胞间通信.
- 外体,细胞外囊泡,在TME信号传递中发挥关键作用,影响癌症的进展.
研究的目的:
- 为了研究从多克索鲁比 (DOX) 处理的巨体和DOX载荷的循环素基纳米海绵 (DOX-CDNSs) 的外体对黑色素瘤癌细胞增殖的影响.
- 从暴露于DOX和DOX-CDNS的RAW 264.7巨细胞中获得的外体的特征.
主要方法:
- 在用DOX和DOX-CDNSs治疗后,从小鼠巨细胞系 (RAW 264.7) 隔离和表征外体.
- 在暴露于不同度的DOX和DOX-CDNS后,评估巨细胞和癌细胞的活力.
- 从经过处理的巨细胞中获得条件介质 (外体) 对癌细胞活力的影响的评估.
主要成果:
- DOX-CDNSs (1μg/mL) 对巨细胞无毒,但对癌细胞有毒,与同样度的单独DOX不同.
- 较高的DOX和DOX-CDNS度 (25和50微克/毫升) 降低了两种细胞类型的活力.
- 来自DOX-CDNSs处理的巨细胞的外体细胞在1,20和40μg/mL时促进了癌细胞活力,而来自DOX处理的巨细胞的外体细胞仅在1μg/mL时表现出这种效果.
结论:
- 循环德克斯特林纳米海绵 (CD-NSs) 可能会增强多克索鲁比的抗癌疗效.
- 巨细胞衍生的外体,特别是受DOX-CDNSs影响的外体,可以矛盾地促进癌细胞的生长,突出显示复杂的TME相互作用.
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