癌基因诱导衰老/SASP替代品在与HPV相关的癌前宫组织中的可变表达
Tareq Saleh1, Nisreen Himsawi2, Amani Al Rousan3
1Department of Pharmacology and Public Health, Faculty of Medicine, The Hashemite University, Zarqa 13133, Jordan.
Current issues in molecular biology
|December 27, 2024
概括
在感染HPV的宫癌前病变中研究了瘤基因诱导衰老 (OIS) 和其分泌表型 (SASP) 标记物. 观察到拉敏B1下调和特定的SASP标记物变化,这表明它在宫癌的发展中发挥了作用.
科学领域:
- 细胞生物学 细胞生物学
- 在瘤学瘤学.
- 病毒学 病毒学
背景情况:
- 瘤基因诱导衰老 (OIS) 和与衰老相关的分泌表型 (SASP) 都与癌症的发展有关.
- 在宫癌中OIS和SASP的作用,特别是在感染人类乳头瘤病毒 (HPV) 的癌前病变中,尚不清楚.
- 在宫癌前病变中研究这些标志物对于理解恶性转变至关重要.
研究的目的:
- 为了研究OIS和SASP标记物在HPV感染的宫癌前病变中的表达.
- 为了确定HPV感染,OIS标记物 (Lamin B1,CDKN2A,CDKN2B) 和SASP标记物 (IL1A,CCL2,TGFB1,CXCL8,MMP9) 之间的相关性.
- 探索OIS和SASP在宫癌进展中的潜在作用.
主要方法:
- 在患者获得的癌前 (n=32) 和非癌前 (n=10) 宫组织样本中使用qRT-PCR对OIS和SASP生物标志物基因表达的分析.
- 通过免疫组织化学染色来确认OIS状态,以检测Lamin B1下调.
- 在癌前病变中检测HPV状态和基因型 (主要是HPV-16).
主要成果:
- 与非癌性组织相比,在癌前病变中观察到Lamin B1 (一种OIS标志物) 的显著下调 (p < 0.001),与HPV E6表达相关.
- 在癌前病变中观察到OIS标记物CDKN2A和CDKN2B的升级.
- 癌前病变中的SASP概况显示CXCL8和TGFB1的升高,IL1A,CCL2和MMP9的表达减少.
结论:
- 拉胺B1的下调和CDKN2A/CDKN2B的改变表达表明HPV感染的宫癌前病变中存在OIS.
- 这些病变与SASP组件 (CXCL8,TGFB1,IL1A,CCL2,MMP9) 的特定变化有关.
- 对OIS和SASP的作用进行进一步的研究是有必要的,以了解宫恶性转变.
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